3jbo

Electron Microscopy
5.8Å resolution

Cryo-electron microscopy reconstruction of the Plasmodium falciparum 80S ribosome bound to P/E-tRNA

Released:
Source organism: Plasmodium falciparum 3D7
Primary publication:
Dynamical features of the Plasmodium falciparum ribosome during translation.
OpenAccess logo Nucleic Acids Res 43 10515-24 (2015)
PMID: 26432834
Related structures: EMD-6452

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 78-mer (preferred)
PDBe Complex ID:
PDB-CPX-249565 (preferred)
Entry contents:
73 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (78 distinct):
40S ribosomal protein S23, putative Chain: Q
Molecule details ›
Chain: Q
Length: 144 amino acids
Theoretical weight: 16.04 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: O97248 (Residues: 2-145; Coverage: 99%)
Gene name: PF3D7_0306900
Sequence domains: Ribosomal protein S12/S23
40S ribosomal protein S18, putative Chain: S
Molecule details ›
Chain: S
Length: 128 amino acids
Theoretical weight: 14.92 KDa
Source organism: Plasmodium falciparum 3D7
UniProt:
  • Canonical: Q8IIA2 (Residues: 12-139; Coverage: 82%)
Gene name: PF3D7_1126200
Sequence domains: Ribosomal protein S13/S18
40S ribosomal protein S29, putative Chain: T
40S ribosomal protein S16, putative Chain: M
Small ribosomal subunit protein uS15 N-terminal domain-containing protein Chain: U
Small ribosomal subunit protein uS17 N-terminal domain-containing protein Chain: V
40S ribosomal protein S9, putative Chain: E
40S ribosomal protein S19 Chain: X
Small ribosomal subunit protein uS5 Chain: G
40S ribosomal protein S17, putative Chain: W
Small ribosomal subunit protein eS12 Chain: R
Small ribosomal subunit protein uS7 domain-containing protein Chain: I
Plectin/eS10 N-terminal domain-containing protein Chain: O
40S ribosomal protein S19 Chain: Y
40S ribosomal protein S21 Chain: Z
40S ribosomal protein S24 Chain: 1
40S ribosomal protein S25 Chain: 2
Small ribosomal subunit protein uS2 Chain: C
40S ribosomal protein S26 Chain: 3
40S ribosomal protein S27 Chain: 4
40S ribosomal protein S28e, putative Chain: 5
Small ribosomal subunit protein eS30 Chain: 6
Small ribosomal subunit protein eS1 Chain: B
40S ribosomal protein S3 Chain: D
40S ribosomal protein S4 Chain: F
40S ribosomal protein S6 Chain: H
40S ribosomal protein S15A, putative Chain: K
40S ribosomal protein S7 Chain: J
Small ribosomal subunit protein uS10 domain-containing protein Chain: N
40S ribosomal protein uS11 Chain: P
40S ribosomal protein S8 Chain: L
60S ribosomal protein L13 Chain: AL
Large ribosomal subunit protein eL24-related N-terminal domain-containing protein Chain: A0
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chain: AO
Large ribosomal subunit protein eL42 Chain: Ai
Ribosomal eL28/Mak16 domain-containing protein Chain: A2
60S ribosomal protein L29 Chain: A4
Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein Chain: A6
60S ribosomal protein L31 Chain: A7
60S ribosomal protein L27 Chain: A1
Large ribosomal subunit protein eL14 domain-containing protein Chain: AN
60S ribosomal protein L32 Chain: A8
60S ribosomal protein L35ae, putative Chain: A9
60S ribosomal protein L34 Chain: Aa
60S ribosomal protein L36 Chain: Ab
60S ribosomal protein L38 Chain: Ad
60S ribosomal protein L39 Chain: Ae
Ubiquitin-like domain-containing protein Chain: Af
Ribosomal protein L15 Chain: AP
Large ribosomal subunit protein eL43 Chain: Ah
60S ribosomal protein eL6 Chain: AI
Ribosomal protein L37 Chain: Ac
60S ribosomal protein L13, putative Chain: AK
60S ribosomal protein L23, putative Chain: AM
Large ribosomal subunit protein uL15/eL18 domain-containing protein Chain: AS
60S ribosomal protein L10, putative Chain: AQ
Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein Chain: AR
60S ribosomal protein L17, putative Chain: AW
Large ribosomal subunit protein uL23 N-terminal domain-containing protein Chain: AY
Large ribosomal subunit protein eL19 domain-containing protein Chain: AT
KOW domain-containing protein Chain: AZ
60S ribosomal protein L35, putative Chain: A3
60S ribosomal protein L7, putative Chain: A5
Large ribosomal subunit protein uL2 C-terminal domain-containing protein Chain: AD
60S ribosomal protein L3 Chain: AE
Large ribosomal subunit protein uL4 C-terminal domain-containing protein Chain: AF
60S ribosomal protein L11a, putative Chain: AG
60S ribosomal protein L18a Chain: AU
Large ribosomal subunit protein uL6 alpha-beta domain-containing protein Chain: AH
60S ribosomal protein L21 Chain: AV
60S ribosomal protein L41 Chain: Ag
Large ribosomal subunit protein eL22 Chain: AX
60S ribosomal protein L7a Chain: AJ
18S Ribosomal RNA Chain: A
P/E-tRNA Chain: 7
28S ribosomal RNA Chain: AA
5.8S ribosomal RNA Chain: AC
5S ribosomal RNA Chain: AB

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.8Å
Relevant EMDB volumes: EMD-6452