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Crystal structure of Fis bound to 27 bp DNA F27 containing a C/G at center

X-ray diffraction
3.05Å resolution
PDB entry 3jrf coloured by chain, front view.
PDB entry 3jrf coloured by chain, side view.
PDB entry 3jrf coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA-binding protein Fis and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 19264.42 Å2
Buried surface area: 8881.63 Å2
Dissociation area: 1,032.51 Å2
Dissociation energy (ΔGdiss): 8.12 kcal/mol
Dissociation entropy (TΔSdiss): 12.02 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-116445

Macromolecules

DNA-binding protein Fis
Chains: A, B
Length: 98 amino acids
Theoretical weight: 11.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6R3 (Residues: 1-98; Coverage: 100%)
Gene names: JW3229, b3261, fis
Pfam: Bacterial regulatory protein, Fis family
InterPro:
CATH: Homeodomain-like
DNA-binding protein Fis in PDB entry 3jrf, assembly 1, front view.
DNA-binding protein Fis in PDB entry 3jrf, assembly 1, side view.
DNA-binding protein Fis in PDB entry 3jrf, assembly 1, top view.
PDBe-KB: UniProt Coverage View: P0A6R3  
198102030405060708090
 
50MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN
UniProt
P0A6R3
Chains
Domains
Secondary structure
Flexibility predictions
Early folding residue predictions
Interaction interfaces
Sequence conservation
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DNA chain C
Name: DNA (27-MER)
Representative chains: C
Source organism: Escherichia coli K-12 [83333]
Expression system: Not provided
Length: 27 nucleotides
Theoretical weight: 8.32 KDa
DNA (27-MER) in PDB entry 3jrf, assembly 1, front view.
DNA (27-MER) in PDB entry 3jrf, assembly 1, side view.
DNA (27-MER) in PDB entry 3jrf, assembly 1, top view.
1272468101214161820222426
 
1020AAATTTGTTTGAACTTTGAGCAAATTT
Chains
RSRZ Outlier Chain C (auth C)
Chain C (auth C)
Interaction interfaces
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DNA chain D
Name: DNA (27-MER)
Representative chains: D
Source organism: Escherichia coli K-12 [83333]
Expression system: Not provided
Length: 27 nucleotides
Theoretical weight: 8.27 KDa
DNA (27-MER) in PDB entry 3jrf, assembly 1, front view.
DNA (27-MER) in PDB entry 3jrf, assembly 1, side view.
DNA (27-MER) in PDB entry 3jrf, assembly 1, top view.
1272468101214161820222426
 
1020AAATTTGCTCAAAGTTCAAACAAATTT
Chains
RSRZ Outlier Chain D (auth D)
Chain D (auth D)
Interaction interfaces
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