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Mint deletion mutant of heterotetrameric geranyl pyrophosphate synthase in complex with ligands

X-ray diffraction
2.3Å resolution
PDB entry 3oab coloured by chain, front view.
PDB entry 3oab coloured by chain, side view.
PDB entry 3oab coloured by chain, top view.
Source organism: Mentha x piperita
Assemblies composition:
hetero tetramer (preferred)
hetero dimer
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Geranyl pyrophosphate synthase large subunit and Gpp synthase small subunit
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Multimeric state: hetero tetramer
Accessible surface area: 41117.22 Å2
Buried surface area: 11582.96 Å2
Dissociation area: 871.34 Å2
Dissociation energy (ΔGdiss): -7.58 kcal/mol
Dissociation entropy (TΔSdiss): 14.69 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-193440
Assembly 2
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Assembly Name: Geranyl pyrophosphate synthase large subunit and Gpp synthase small subunit
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Multimeric state: hetero dimer
Accessible surface area: 21914.78 Å2
Buried surface area: 4660.06 Å2
Dissociation area: 1,811.14 Å2
Dissociation energy (ΔGdiss): 27.82 kcal/mol
Dissociation entropy (TΔSdiss): 13.05 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-193439
Assembly 3
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Assembly Name: Geranyl pyrophosphate synthase large subunit and Gpp synthase small subunit
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Multimeric state: hetero dimer
Accessible surface area: 20932.89 Å2
Buried surface area: 5192.45 Å2
Dissociation area: 1,773.93 Å2
Dissociation energy (ΔGdiss): 26.84 kcal/mol
Dissociation entropy (TΔSdiss): 13.01 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-193439

Macromolecules

Gpp synthase large subunit
Chains: A, D
Length: 295 amino acids
Theoretical weight: 31.95 KDa
Source organism: Mentha x piperita
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SBR3 (Residues: 84-377; Coverage: 78%)
Pfam: Polyprenyl synthetase
InterPro:
CATH: Farnesyl Diphosphate Synthase
Geranyl pyrophosphate synthase large subunit in PDB entry 3oab, assembly 1, front view.
Geranyl pyrophosphate synthase large subunit in PDB entry 3oab, assembly 1, side view.
Geranyl pyrophosphate synthase large subunit in PDB entry 3oab, assembly 1, top view.
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Geranyl diphosphate synthase small subunit
Chains: B, C
Length: 264 amino acids
Theoretical weight: 28.71 KDa
Source organism: Mentha x piperita
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SBR4 (Residues: 49-313; Coverage: 82%)
InterPro: Isoprenoid synthase domain superfamily
CATH: Farnesyl Diphosphate Synthase
Gpp synthase small subunit in PDB entry 3oab, assembly 1, front view.
Gpp synthase small subunit in PDB entry 3oab, assembly 1, side view.
Gpp synthase small subunit in PDB entry 3oab, assembly 1, top view.
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