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Crystal structure of putrescine transcarbamylase from Enterococcus faecalis with N5-(phosphonoacetyl)-L-ornithine

X-ray diffraction
2Å resolution
PDB entry 4a8p coloured by chain, front view.
PDB entry 4a8p coloured by chain, side view.
PDB entry 4a8p coloured by chain, top view.
Source organism: Enterococcus faecalis
Assembly composition:
homo trimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Putrescine carbamoyltransferase
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Multimeric state: homo trimer
Accessible surface area: 35504.86 Å2
Buried surface area: 12407.54 Å2
Dissociation area: 5,942.14 Å2
Dissociation energy (ΔGdiss): 59.95 kcal/mol
Dissociation entropy (TΔSdiss): 29.1 kcal/mol
Symmetry number: 3
PDBe Complex ID: PDB-CPX-182851
Assembly 2
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Assembly Name: Putrescine carbamoyltransferase
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Multimeric state: homo trimer
Accessible surface area: 35269.83 Å2
Buried surface area: 10503.76 Å2
Dissociation area: 5,251.88 Å2
Dissociation energy (ΔGdiss): 54.69 kcal/mol
Dissociation entropy (TΔSdiss): 28.74 kcal/mol
Symmetry number: 3
PDBe Complex ID: PDB-CPX-182851

Macromolecules

Putrescine carbamoyltransferase
Chains: A, B, C, D, E, F
Length: 355 amino acids
Theoretical weight: 40.14 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q837U7 (Residues: 1-339; Coverage: 100%)
Gene names: EF_0732, agcB, argF-2, ptcA
Pfam:
InterPro:
CATH: Aspartate/ornithine carbamoyltransferase
Putrescine carbamoyltransferase in PDB entry 4a8p, assembly 1, front view.
Putrescine carbamoyltransferase in PDB entry 4a8p, assembly 1, side view.
Putrescine carbamoyltransferase in PDB entry 4a8p, assembly 1, top view.
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