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Crystal structure of a putative methylthioadenosine nucleosidase from Weissella paramesenteroides ATCC 33313 (Target NYSGRC-029342 )

X-ray diffraction
1.7Å resolution
PDB entry 4kn5 coloured by chain, front view.
PDB entry 4kn5 coloured by chain, side view.
PDB entry 4kn5 coloured by chain, top view.
Source organism: Weissella paramesenteroides ATCC 33313
Assembly composition:
homo dimer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: adenosylhomocysteine nucleosidase
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Multimeric state: homo dimer
Accessible surface area: 18300.96 Å2
Buried surface area: 3880.33 Å2
Dissociation area: 1,456.34 Å2
Dissociation energy (ΔGdiss): 13.06 kcal/mol
Dissociation entropy (TΔSdiss): 12.97 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-111533

Macromolecules

adenosylhomocysteine nucleosidase
Chains: A, B
Length: 253 amino acids
Theoretical weight: 27.44 KDa
Source organism: Weissella paramesenteroides ATCC 33313
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C5RAK4 (Residues: 2-231; Coverage: 100%)
Gene names: HMPREF0877_0999, mtnN
Pfam: Phosphorylase superfamily
InterPro:
CATH: Nucleoside phosphorylase domain
adenosylhomocysteine nucleosidase in PDB entry 4kn5, assembly 1, front view.
adenosylhomocysteine nucleosidase in PDB entry 4kn5, assembly 1, side view.
adenosylhomocysteine nucleosidase in PDB entry 4kn5, assembly 1, top view.
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