Structure analysis

Structure of a SecM-stalled E. coli ribosome complex obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1143

Electron Microscopy
15Å resolution
Source organism: Escherichia coli
Assembly composition:
hetero 52-mer (preferred)
Entry contents: 45 distinct polypeptide molecules
4 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 52-mer
Accessible surface area: 942415.93 Å2
Buried surface area: 145079.53 Å2
Dissociation area: 2,765.81 Å2
Dissociation energy (ΔGdiss): -36.33 kcal/mol
Dissociation entropy (TΔSdiss): 84.68 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-135579

Macromolecules

PDBe-KB: UniProt Coverage View: P0A7V0  
123620406080100120140160180200220
 
100200
UniProt
P0A7V0
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PDBe-KB: UniProt Coverage View: P0A7V3  
120620406080100120140160180200
 
50100150200
UniProt
P0A7V3
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PDBe-KB: UniProt Coverage View: P0A7V8  
120420406080100120140160180200
 
50100150200
UniProt
P0A7V8
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PDBe-KB: UniProt Coverage View: P0A7W1  
114820406080100120140
 
50100
UniProt
P0A7W1
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PDBe-KB: UniProt Coverage View: P02358  
195102030405060708090
 
50MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGRRQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHA
UniProt
P02358
Chains
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PDBe-KB: UniProt Coverage View: P02359  
1137102030405060708090100110120130
 
50100
UniProt
P02359
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Chain: AH
Length: 127 amino acids
Theoretical weight: 13.86 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7W7 (Residues: 3-129; Coverage: 98%)
Gene names: JW3268, b3306, rpsH
Pfam: Ribosomal protein S8
InterPro:
PDBe-KB: UniProt Coverage View: P0A7W7  
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PDBe-KB: UniProt Coverage View: P0A7X3  
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PDBe-KB: UniProt Coverage View: P0A7R5  
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Chain: AK
Length: 116 amino acids
Theoretical weight: 12.39 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7R9 (Residues: 13-128; Coverage: 90%)
Gene names: JW3259, b3297, rpsK
Pfam: Ribosomal protein S11
InterPro:
PDBe-KB: UniProt Coverage View: P0A7R9  
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Chain: AL
Length: 101 amino acids
Theoretical weight: 11.25 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 23-123; Coverage: 82%)
Gene names: JW3304, b3342, rpsL, strA
Pfam: Ribosomal protein S12/S23
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PDBe-KB: UniProt Coverage View: P0A7S9  
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Chain: AN
Length: 61 amino acids
Theoretical weight: 7.12 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG59 (Residues: 41-101; Coverage: 60%)
Gene names: JW3269, b3307, rpsN
Pfam: Ribosomal protein S14p/S29e
InterPro:
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Chain: AO
Length: 86 amino acids
Theoretical weight: 9.92 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 3-88; Coverage: 97%)
Gene names: JW3134, b3165, rpsO, secC
Pfam: Ribosomal protein S15
InterPro:
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Chain: AP
Length: 78 amino acids
Theoretical weight: 8.82 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T3 (Residues: 1-78; Coverage: 95%)
Gene names: JW2590, b2609, rpsP
Pfam: Ribosomal protein S16
InterPro:
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Chain: AQ
Length: 79 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG63 (Residues: 6-84; Coverage: 94%)
Gene names: JW3273, b3311, neaA, rpsQ
Pfam: Ribosomal protein S17
InterPro:
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Chain: AR
Length: 69 amino acids
Theoretical weight: 8.18 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7T7 (Residues: 7-75; Coverage: 92%)
Gene names: JW4160, b4202, rpsR
Pfam: Ribosomal protein S18
InterPro:
PDBe-KB: UniProt Coverage View: P0A7T7  
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Chain: AS
Length: 87 amino acids
Theoretical weight: 9.87 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U3 (Residues: 2-88; Coverage: 95%)
Gene names: JW3278, b3316, rpsS
Pfam: Ribosomal protein S19
InterPro:
PDBe-KB: UniProt Coverage View: P0A7U3  
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Chain: AT
Length: 83 amino acids
Theoretical weight: 9.28 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7U7 (Residues: 5-87; Coverage: 95%)
Gene names: JW0022, b0023, rpsT
Pfam: Ribosomal protein S20
InterPro:
PDBe-KB: UniProt Coverage View: P0A7U7  
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PDBe-KB: UniProt Coverage View: P0A7L0  
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PDBe-KB: UniProt Coverage View: P60438  
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Chain: BC
Length: 198 amino acids
Theoretical weight: 21.81 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P60723 (Residues: 1-198; Coverage: 99%)
Gene names: JW3281, b3319, eryA, rplD
Pfam: Ribosomal protein L4/L1 family
InterPro:
PDBe-KB: UniProt Coverage View: P60723  
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PDBe-KB: UniProt Coverage View: P62399  
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PDBe-KB: UniProt Coverage View: P0AG55  
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Chain: BH
Length: 142 amino acids
Theoretical weight: 16.05 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AA10 (Residues: 1-142; Coverage: 100%)
Gene names: JW3200, b3231, rplM
Pfam: Ribosomal protein L13
InterPro:
PDBe-KB: UniProt Coverage View: P0AA10  
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Chain: BI
Length: 122 amino acids
Theoretical weight: 13.43 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0ADY3 (Residues: 2-123; Coverage: 99%)
Gene names: JW3272, b3310, rplN
Pfam: Ribosomal protein L14p/L23e
InterPro:
PDBe-KB: UniProt Coverage View: P0ADY3  
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PDBe-KB: UniProt Coverage View: P02413  
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PDBe-KB: UniProt Coverage View: P0ADY7  
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Chain: BL
Length: 114 amino acids
Theoretical weight: 12.95 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0AG44 (Residues: 3-116; Coverage: 90%)
Gene names: JW3256, b3294, rplQ
Pfam: Ribosomal protein L17
InterPro:
PDBe-KB: UniProt Coverage View: P0AG44  
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Chain: BM
Length: 113 amino acids
Theoretical weight: 12.27 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0C018 (Residues: 3-115; Coverage: 97%)
Gene names: JW3266, b3304, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
InterPro:
PDBe-KB: UniProt Coverage View: P0C018  
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Chain: BN
Length: 114 amino acids
Theoretical weight: 13.03 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7K6 (Residues: 2-115; Coverage: 99%)
Gene names: JW2587, b2606, rplS
Pfam: Ribosomal protein L19
InterPro:
PDBe-KB: UniProt Coverage View: P0A7K6  
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Chain: BO
Length: 115 amino acids
Theoretical weight: 13.25 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L3 (Residues: 3-117; Coverage: 98%)
Gene names: JW1706, b1716, pdzA, rplT
Pfam: Ribosomal protein L20
InterPro:
PDBe-KB: UniProt Coverage View: P0A7L3  
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PDBe-KB: UniProt Coverage View: P61175  
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PDBe-KB: UniProt Coverage View: P0ADZ0  
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PDBe-KB: UniProt Coverage View: P68919  
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Chain: BU
Length: 84 amino acids
Theoretical weight: 9.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L8 (Residues: 2-85; Coverage: 99%)
Gene names: JW3152, b3185, rpmA
Pfam: Ribosomal L27 protein
InterPro:
PDBe-KB: UniProt Coverage View: P0A7L8  
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Chain: BW
Length: 60 amino acids
Theoretical weight: 7.09 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7M6 (Residues: 1-60; Coverage: 95%)
Gene names: JW3274, b3312, rpmC
Pfam: Ribosomal L29 protein
InterPro:
PDBe-KB: UniProt Coverage View: P0A7M6  
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Chain: BZ
Length: 29 amino acids
Theoretical weight: 3.32 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N4 (Residues: 28-56; Coverage: 51%)
Gene names: JW1075, b1089, rpmF
Pfam: Ribosomal L32p protein family
InterPro:
PDBe-KB: UniProt Coverage View: P0A7N4  
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Chain: B1
Length: 52 amino acids
Theoretical weight: 6.02 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N9 (Residues: 2-53; Coverage: 95%)
Gene names: JW3611, b3636, rpmG
Pfam: Ribosomal protein L33
InterPro:
PDBe-KB: UniProt Coverage View: P0A7N9  
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Name: 16S ribosomal RNA
Representative chains: AA
Length: 1488 nucleotides
Theoretical weight: 482.29 KDa
Rfam: Bacterial small subunit ribosomal RNA
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Name: tRNA
Representative chains: AU, AV, AW
Length: 76 nucleotides
Theoretical weight: 24.52 KDa
Rfam: tRNA
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Name: 23S ribosomal RNA
Representative chains: B0
Length: 2740 nucleotides
Theoretical weight: 888.5 KDa
Rfam: Bacterial large subunit ribosomal RNA
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Name: 5S ribosomal RNA
Representative chains: B9
Length: 108 nucleotides
Theoretical weight: 34.92 KDa
Rfam: 5S ribosomal RNA
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