› › Structure analysis

Crystal structure of Thermus thermophilus CarH bound to adenosylcobalamin and a 26-bp DNA segment

X-ray diffraction
3.89Å resolution
PDB entry 5c8e coloured by chain, front view.
PDB entry 5c8e coloured by chain, side view.
PDB entry 5c8e coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Assembly composition:
hetero hexamer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Assembly Name: Probable transcriptional regulator and DNA
hide full assembly name
Multimeric state: hetero hexamer
Accessible surface area: 46489.52 Å2
Buried surface area: 26781.63 Å2
Dissociation area: 1,742.79 Å2
Dissociation energy (ΔGdiss): 25.78 kcal/mol
Dissociation entropy (TΔSdiss): 13.49 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-118463
Assembly 2
Download    3D Visualisation
Assembly Name: Probable transcriptional regulator and DNA
hide full assembly name
Multimeric state: hetero hexamer
Accessible surface area: 46974.69 Å2
Buried surface area: 26243.78 Å2
Dissociation area: 1,697.45 Å2
Dissociation energy (ΔGdiss): 22.79 kcal/mol
Dissociation entropy (TΔSdiss): 13.47 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-118463

Macromolecules

Probable transcriptional regulator
Chains: A, B, C, D, E, F, G, H
Length: 305 amino acids
Theoretical weight: 33.32 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746J7 (Residues: 1-285; Coverage: 100%)
Gene name: TT_P0056
Pfam:
InterPro:
CATH:
Probable transcriptional regulator in PDB entry 5c8e, assembly 1, front view.
Probable transcriptional regulator in PDB entry 5c8e, assembly 1, side view.
Probable transcriptional regulator in PDB entry 5c8e, assembly 1, top view.
PDBe-KB: UniProt Coverage View: Q746J7  
130520406080100120140160180200220240260280300
 
100200300
UniProt
Q746J7
Chains
Domains
Secondary structure
Flexibility predictions
Early folding residue predictions
Ligand binding sites
Interaction interfaces
Molecule details ›

Search similar proteins

DNA chains I, K
Name: 26-mer DNA segment containing the CarH operator sequence (antisense strand)
Representative chains: I, K
Source organism: Thermus thermophilus HB27 [262724]
Expression system: Not provided
Length: 26 nucleotides
Theoretical weight: 8 KDa
26-mer DNA segment containing the CarH operator sequence (antisense strand) in PDB entry 5c8e, assembly 1, front view.
26-mer DNA segment containing the CarH operator sequence (antisense strand) in PDB entry 5c8e, assembly 1, side view.
26-mer DNA segment containing the CarH operator sequence (antisense strand) in PDB entry 5c8e, assembly 1, top view.
1262468101214161820222426
 
1020
Chains
Chain I (auth I)
Chain K (auth K)
Interaction interfaces
Molecule details ›

Search similar DNA

DNA chains J, L
Name: 26-mer DNA segment containing the CarH operator sequence (sense strand)
Representative chains: J, L
Source organism: Thermus thermophilus HB27 [262724]
Expression system: Not provided
Length: 26 nucleotides
Theoretical weight: 7.97 KDa
26-mer DNA segment containing the CarH operator sequence (sense strand) in PDB entry 5c8e, assembly 1, front view.
26-mer DNA segment containing the CarH operator sequence (sense strand) in PDB entry 5c8e, assembly 1, side view.
26-mer DNA segment containing the CarH operator sequence (sense strand) in PDB entry 5c8e, assembly 1, top view.
1262468101214161820222426
 
1020
Chains
Chain J (auth J)
Chain L (auth L)
Interaction interfaces
Molecule details ›

Search similar DNA

Quick links