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Aminoglycoside Phosphotransferase (2'')-Ia (CTD of AAC(6')-Ie/APH(2'')-Ia) in complex with GMPPNP and Magnesium

X-ray diffraction
2.15Å resolution
PDB entry 5iqa coloured by chain, front view.
PDB entry 5iqa coloured by chain, side view.
PDB entry 5iqa coloured by chain, top view.
Source organism: Staphylococcus aureus
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Bifunctional AAC/APH
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Multimeric state: monomeric
Accessible surface area: 15222.38 Å2
Buried surface area: 1264.19 Å2
Dissociation area: 81.21 Å2
Dissociation energy (ΔGdiss): 12.03 kcal/mol
Dissociation entropy (TΔSdiss): -1.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-140902
Assembly 2
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Assembly Name: Bifunctional AAC/APH
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Multimeric state: monomeric
Accessible surface area: 15567.47 Å2
Buried surface area: 1266.65 Å2
Dissociation area: 80.7 Å2
Dissociation energy (ΔGdiss): 12.02 kcal/mol
Dissociation entropy (TΔSdiss): -1.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-140902
Assembly 3
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Assembly Name: Bifunctional AAC/APH
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Multimeric state: monomeric
Accessible surface area: 15490.41 Å2
Buried surface area: 1250.27 Å2
Dissociation area: 81.7 Å2
Dissociation energy (ΔGdiss): 12.09 kcal/mol
Dissociation entropy (TΔSdiss): -1.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-140902
Assembly 4
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Assembly Name: Bifunctional AAC/APH
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Multimeric state: monomeric
Accessible surface area: 15655.27 Å2
Buried surface area: 1090.03 Å2
Dissociation area: 80.28 Å2
Dissociation energy (ΔGdiss): 12.56 kcal/mol
Dissociation entropy (TΔSdiss): -1.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-140902

Macromolecules

Bifunctional AAC/APH
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 35.95 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A0C1 (Residues: 175-479; Coverage: 64%)
Gene names: R015, VRA0030, aacA-aphD
Pfam: Phosphotransferase enzyme family
InterPro:
CATH:
Bifunctional AAC/APH in PDB entry 5iqa, assembly 1, front view.
Bifunctional AAC/APH in PDB entry 5iqa, assembly 1, side view.
Bifunctional AAC/APH in PDB entry 5iqa, assembly 1, top view.
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