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The LPS Transporter LptDE from Yersinia pestis, core complex

X-ray diffraction
2.746Å resolution
PDB entry 5ixm coloured by chain, front view.
PDB entry 5ixm coloured by chain, side view.
PDB entry 5ixm coloured by chain, top view.
Source organism: Yersinia pestis
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1
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Assembly Name: LPS-assembly lipoprotein LptE and LPS-assembly protein LptD
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Multimeric state: hetero dimer
Accessible surface area: 33559.71 Å2
Buried surface area: 8791.03 Å2
Dissociation area: 3,178.18 Å2
Dissociation energy (ΔGdiss): 21.88 kcal/mol
Dissociation entropy (TΔSdiss): 13.53 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-181390
Assembly 2
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Assembly Name: LPS-assembly lipoprotein LptE and LPS-assembly protein LptD
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Multimeric state: hetero dimer
Accessible surface area: 34479.02 Å2
Buried surface area: 10682.77 Å2
Dissociation area: 3,348.22 Å2
Dissociation energy (ΔGdiss): 22 kcal/mol
Dissociation entropy (TΔSdiss): 13.6 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-181390
Assembly 3
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Assembly Name: LPS-assembly lipoprotein LptE and LPS-assembly protein LptD
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Multimeric state: hetero dimer
Accessible surface area: 34248.57 Å2
Buried surface area: 7936.59 Å2
Dissociation area: 3,113.82 Å2
Dissociation energy (ΔGdiss): 23.22 kcal/mol
Dissociation entropy (TΔSdiss): 13.53 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-181390
Assembly 4 (preferred)
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Assembly Name: LPS-assembly lipoprotein LptE and LPS-assembly protein LptD
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Multimeric state: hetero dimer
Accessible surface area: 33741.75 Å2
Buried surface area: 7875.92 Å2
Dissociation area: 3,350.45 Å2
Dissociation energy (ΔGdiss): 21.67 kcal/mol
Dissociation entropy (TΔSdiss): 13.62 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-181390

Macromolecules

LPS-assembly protein LptD
Chains: A, C, E, G
Length: 577 amino acids
Theoretical weight: 65.63 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZIK3 (Residues: 225-780; Coverage: 74%)
Gene names: YPO0495, YP_3684, imp, lptD, ostA, y3680
Pfam: LPS transport system D
InterPro: LptD, C-terminal
LPS-assembly protein LptD in PDB entry 5ixm, assembly 4, front view.
LPS-assembly protein LptD in PDB entry 5ixm, assembly 4, side view.
LPS-assembly protein LptD in PDB entry 5ixm, assembly 4, top view.
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LPS-assembly lipoprotein LptE
Chains: B, D, F, H
Length: 198 amino acids
Theoretical weight: 21.76 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7CJV2 (Residues: 21-207; Coverage: 100%)
Gene names: YPO2609, YP_1104, lptE, rlpB, y1183
Pfam: Lipopolysaccharide-assembly
InterPro: LPS-assembly lipoprotein LptE
CATH: Lipoprotein like domain
LPS-assembly lipoprotein LptE in PDB entry 5ixm, assembly 4, front view.
LPS-assembly lipoprotein LptE in PDB entry 5ixm, assembly 4, side view.
LPS-assembly lipoprotein LptE in PDB entry 5ixm, assembly 4, top view.
Molecule details ›

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