› 5jek

X-ray diffraction
2.4Å resolution

Phosphorylated MAVS in complex with IRF-3

Released:
DOI: 10.2210/pdb5jek/pdb
PDB entry 5jek coloured by chain, front view.
PDB entry 5jek coloured by chain, side view.
PDB entry 5jek coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for concerted recruitment and activation of IRF-3 by innate immune adaptor proteins.
Zhao B, Shu C, Gao X, Sankaran B, Du F, Shelton CL, Herr AB, Ji JY, Li P
Proc Natl Acad Sci U S A 113 E3403-12 (2016)
PMID: 27302953

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172050 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Interferon regulatory factor 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 242 amino acids
Theoretical weight: 26.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14653 (Residues: 189-427; Coverage: 56%)
Gene name: IRF3
Sequence domains: Interferon-regulatory factor 3
Structure domains: Tumour Suppressor Smad4
Mitochondrial antiviral-signaling protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 18 amino acids
Theoretical weight: 1.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z434 (Residues: 433-450; Coverage: 3%)
Gene names: IPS1, KIAA1271, MAVS, VISA

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 38.8Å b: 104.91Å c: 66.27Å
α: 90° β: 106.13° γ: 90°
R-values:
R R work R free
0.194 0.188 0.251
Expression system: Escherichia coli

Quick links

Citations

33 review citations

Molecular mechanisms and cellular functions of cGAS-STING signalling.
Hopfner et al. (2020)
32 more

3 mentions without citation

Structural basis for concerted recruitment and activation of IRF-3 by innate immune adaptor proteins.
Zhao et al. (2016)
2 more