5kgf

Electron Microscopy
4.54Å resolution

Structural model of 53BP1 bound to a ubiquitylated and methylated nucleosome, at 4.5 A resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-118946 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
Histone H3.2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.42 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 1-136; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.44 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 1-103; Coverage: 100%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chains: C, G
Histone H2B type 1-C/E/F/G/I Chains: D, H
TP53-binding protein 1 Chains: K, L
Ubiquitin Chains: M, O
DNA (145-MER) Chain: I
DNA (145-MER) Chain: J

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Resolution: 4.54Å
Relevant EMDB volumes: EMD-8246
Expression system: Escherichia coli