Sample information
Author description:
Structural model of 53BP1 bound to a ubiquitylated and methylated nucleosome, at 4.5 A resolution
Source organisms:
Expression system:
Escherichia coli
Validation information
Experimental information
Specimen preparation
| Buffer name | pH | Details |
|---|---|---|
| - | 7.5 | High concentration NCP-ubme/GST-53BP1 complex at 200 mM salt was diluted just prior to grid freezing. |
Vitrification
| Cryogen | Temperature | Instrument | Humidity | Details |
|---|---|---|---|---|
| - | - | FEI VITROBOT MARK III | 100 | Plunged into liquid ethane-propane (FEI VITROBOT MARK III) |
Refinement statistics
Structure solution method:
Electron Microscopy
Resolution:
4.54 Å
