5n61

Electron Microscopy
3.4Å resolution

RNA polymerase I initially transcribing complex

Released:
Related structures: EMD-3593

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
PDBe Complex ID:
PDB-CPX-119044 (preferred)
Entry contents:
18 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (21 distinct):
DNA-directed RNA polymerase I subunit RPA190 Chain: A
Molecule details ›
Chain: A
Length: 1664 amino acids
Theoretical weight: 186.68 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P10964 (Residues: 1-1664; Coverage: 100%)
Gene names: O6276, RPA1, RPA190, RRN1, YOR341W
Sequence domains:
DNA-directed RNA polymerases I and III subunit RPAC1 Chain: C
DNA-directed RNA polymerase I subunit RPA14 Chain: D
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerase I subunit RPA43 Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase I subunit RPA12 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerases I and III subunit RPAC2 Chain: K
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
DNA-directed RNA polymerase I subunit RPA49 Chain: M
DNA-directed RNA polymerase I subunit RPA34 Chain: N
RNA polymerase I-specific transcription initiation factor RRN3 Chain: O
RNA polymerase I-specific transcription initiation factor RRN6 Chain: P
RNA polymerase I-specific transcription initiation factor RRN7 Chain: Q
RNA polymerase I-specific transcription initiation factor RRN11 Chain: R
template DNA Chain: T
non-template DNA Chain: U
product RNA Chain: S

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-3593
Expression system: Escherichia coli BL21(DE3)