› › Structure analysis

Clostridium difficile Toxin B (TcdB) glucosyltransferase domain co-crystallized with apigenin

X-ray diffraction
2.75Å resolution
PDB entry 5uqt coloured by chain, front view.
PDB entry 5uqt coloured by chain, side view.
PDB entry 5uqt coloured by chain, top view.
Source organism: Clostridioides difficile
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: Glucosyltransferase TcdB
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Multimeric state: monomeric
Accessible surface area: 25424.01 Å2
Buried surface area: 134.8 Å2
Dissociation area: 67.4 Å2
Dissociation energy (ΔGdiss): 5.08 kcal/mol
Dissociation entropy (TΔSdiss): -0.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148298
Assembly 2 (preferred)
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Assembly Name: Glucosyltransferase TcdB
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Multimeric state: monomeric
Accessible surface area: 25540.48 Å2
Buried surface area: 132.33 Å2
Dissociation area: 66.16 Å2
Dissociation energy (ΔGdiss): 5.11 kcal/mol
Dissociation entropy (TΔSdiss): -0.13 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-148298

Macromolecules

Glucosyltransferase TcdB
Chains: A, B
Length: 563 amino acids
Theoretical weight: 65.36 KDa
Source organism: Clostridioides difficile
Expression system: Escherichia coli
UniProt:
  • Canonical: P18177 (Residues: 1-543; Coverage: 23%)
Gene names: tcdB, toxB
Pfam:
InterPro:
CATH:
Glucosyltransferase TcdB in PDB entry 5uqt, assembly 2, front view.
Glucosyltransferase TcdB in PDB entry 5uqt, assembly 2, side view.
Glucosyltransferase TcdB in PDB entry 5uqt, assembly 2, top view.
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