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Transition state structure of Cpf1(Cas12a) I4 conformation

Electron Microscopy
3.27Å resolution
PDB entry 6gtg coloured by chain, front view.
PDB entry 6gtg coloured by chain, side view.
PDB entry 6gtg coloured by chain, top view.
Source organism: Francisella tularensis subsp. novicida U112
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: CRISPR-associated endonuclease Cas12a and RNA and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 69435.62 Å2
Buried surface area: 19452.34 Å2
Dissociation area: 1,653.72 Å2
Dissociation energy (ΔGdiss): 21.05 kcal/mol
Dissociation entropy (TΔSdiss): 11.82 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-106193

Macromolecules

CRISPR-associated endonuclease Cas12a
Chain: A
Length: 1329 amino acids
Theoretical weight: 155.64 KDa
Source organism: Francisella tularensis subsp. novicida U112
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0Q7Q2 (Residues: 1-1300; Coverage: 100%)
Gene names: FTN_1397, cas12a, cpf1
Pfam:
InterPro:
CRISPR-associated endonuclease Cas12a in PDB entry 6gtg, assembly 1, front view.
CRISPR-associated endonuclease Cas12a in PDB entry 6gtg, assembly 1, side view.
CRISPR-associated endonuclease Cas12a in PDB entry 6gtg, assembly 1, top view.
PDBe-KB: UniProt Coverage View: A0Q7Q2  
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DNA chain C
Name: DNA (32-MER)
Representative chains: C
Source organism: Francisella tularensis subsp. novicida U112 [401614]
Expression system: Not provided
Length: 55 nucleotides
Theoretical weight: 16.9 KDa
DNA (32-MER) in PDB entry 6gtg, assembly 1, front view.
DNA (32-MER) in PDB entry 6gtg, assembly 1, side view.
DNA (32-MER) in PDB entry 6gtg, assembly 1, top view.
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DNA chain D
Name: DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3')
Representative chains: D
Source organism: Francisella tularensis subsp. novicida U112 [401614]
Expression system: Not provided
Length: 55 nucleotides
Theoretical weight: 16.99 KDa
DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3') in PDB entry 6gtg, assembly 1, front view.
DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3') in PDB entry 6gtg, assembly 1, side view.
DNA (5'-D(P*CP*GP*AP*GP*CP*TP*CP*GP*TP*TP*AP*GP*AP*GP*AP*AP*GP*T)-3') in PDB entry 6gtg, assembly 1, top view.
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RNA chain B
Name: RNA (40-MER)
Representative chains: B
Source organism: Francisella tularensis subsp. novicida U112 [401614]
Expression system: Not provided
Length: 43 nucleotides
Theoretical weight: 13.75 KDa
RNA (40-MER) in PDB entry 6gtg, assembly 1, front view.
RNA (40-MER) in PDB entry 6gtg, assembly 1, side view.
RNA (40-MER) in PDB entry 6gtg, assembly 1, top view.
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