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Structure of Influenza Hemagglutinin ectodomain (A/duck/Alberta/35/76) in complex with FISW84 Fab Fragment

Electron Microscopy
3.3Å resolution
PDB entry 6hjp coloured by chain, front view.
PDB entry 6hjp coloured by chain, side view.
PDB entry 6hjp coloured by chain, top view.
Source organisms:
Assembly composition:
hetero dodecamer (preferred)
Entry contents: 4 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Hemagglutinin HA1 chain and IG-heavy chain and IG-light kappa chain
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Multimeric state: hetero dodecamer
Accessible surface area: 128986.4 Å2
Buried surface area: 52277.32 Å2
Dissociation area: 2,373.95 Å2
Dissociation energy (ΔGdiss): -14.36 kcal/mol
Dissociation entropy (TΔSdiss): 45.22 kcal/mol
Symmetry number: 3
PDBe Complex ID: PDB-CPX-279887

Macromolecules

Hemagglutinin HA1 chain
Chains: A, C, E
Length: 324 amino acids
Theoretical weight: 35.68 KDa
Source organism: Influenza A virus (A/mallard/Alberta/35/1976(H1N1))
UniProt:
  • Canonical: P26562 (Residues: 18-341; Coverage: 59%)
Gene name: HA
Pfam: Haemagglutinin
InterPro:
CATH:
Hemagglutinin in PDB entry 6hjp, assembly 1, front view.
Hemagglutinin in PDB entry 6hjp, assembly 1, side view.
Hemagglutinin in PDB entry 6hjp, assembly 1, top view.
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Hemagglutinin HA2 chain
Chains: B, D, F
Length: 174 amino acids
Theoretical weight: 19.95 KDa
Source organism: Influenza A virus (A/mallard/Alberta/35/1976(H1N1))
UniProt:
  • Canonical: P26562 (Residues: 345-518; Coverage: 32%)
Gene name: HA
Pfam: Haemagglutinin
InterPro:
CATH: Hemagglutinin Ectodomain; Chain B
Hemagglutinin in PDB entry 6hjp, assembly 1, front view.
Hemagglutinin in PDB entry 6hjp, assembly 1, side view.
Hemagglutinin in PDB entry 6hjp, assembly 1, top view.
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Heavy chain of FISW84 Fab Fragment
Chains: G, I, K
Length: 216 amino acids
Theoretical weight: 22.93 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
InterPro:
Heavy chain of FISW84 Fab Fragment in PDB entry 6hjp, assembly 1, front view.
Heavy chain of FISW84 Fab Fragment in PDB entry 6hjp, assembly 1, side view.
Heavy chain of FISW84 Fab Fragment in PDB entry 6hjp, assembly 1, top view.
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Light chain of FISW84 Fab Fragment
Chains: H, J, L
Length: 214 amino acids
Theoretical weight: 23.28 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
InterPro:
CATH: Immunoglobulins
Light chain of FISW84 Fab Fragment in PDB entry 6hjp, assembly 1, front view.
Light chain of FISW84 Fab Fragment in PDB entry 6hjp, assembly 1, side view.
Light chain of FISW84 Fab Fragment in PDB entry 6hjp, assembly 1, top view.
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