Structure analysis

Yeast RNA polymerase I elongation complex bound to nucleotide analog GMPCPP (core focused)

Electron Microscopy
3.18Å resolution
Assembly composition:
hetero pentadecamer (preferred)
Entry contents: 12 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero pentadecamer
Accessible surface area: 158741.43 Å2
Buried surface area: 74166.74 Å2
Dissociation area: 2,101.29 Å2
Dissociation energy (ΔGdiss): 9.22 kcal/mol
Dissociation entropy (TΔSdiss): 11.32 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-119994

Macromolecules

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Chain: D
Length: 137 amino acids
Theoretical weight: 14.6 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P50106 (Residues: 1-137; Coverage: 100%)
Gene names: RPA14, YD8358.11, YDR156W
Pfam: Yeast RNA polymerase I subunit RPA14
InterPro: RNA polymerase I, subunit Rpa14, fungi
CATH: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

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Chain: H
Length: 146 amino acids
Theoretical weight: 16.53 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P20436 (Residues: 1-146; Coverage: 100%)
Gene names: RPB8, YOR224C, YOR50-14
Pfam: RNA polymerase Rpb8
InterPro:
CATH: Nucleic acid-binding proteins

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Chain: J
Length: 70 amino acids
Theoretical weight: 8.29 KDa
Source organism: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P22139 (Residues: 1-70; Coverage: 100%)
Gene names: RPB10, YOR210W
Pfam: RNA polymerases N / 8 kDa subunit
InterPro:
CATH: Homeodomain-like

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Name: Non-template strand
Representative chains: S
Length: 38 nucleotides
Theoretical weight: 11.76 KDa

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Name: Template strand
Representative chains: T
Length: 38 nucleotides
Theoretical weight: 11.63 KDa

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Name: RNA
Representative chains: R
Length: 20 nucleotides
Theoretical weight: 6.41 KDa

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