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Ligand-triggered allosteric ADP release primes a plant NLR complex

Electron Microscopy
4.25Å resolution
PDB entry 6j5v coloured by chain, front view.
PDB entry 6j5v coloured by chain, side view.
PDB entry 6j5v coloured by chain, top view.
Source organism: Arabidopsis thaliana
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
PDBe Complex ID: PDB-CPX-129278

Macromolecules

Disease resistance RPP13-like protein 4
Chain: A
Length: 852 amino acids
Theoretical weight: 97.16 KDa
Source organism: Arabidopsis thaliana
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q38834 (Residues: 1-852; Coverage: 100%)
Gene names: At3g50950, F18B3.230, RPP13L4, ZAR1
Pfam:
InterPro:
Disease resistance RPP13-like protein 4 in PDB entry 6j5v, assembly 1, front view.
Disease resistance RPP13-like protein 4 in PDB entry 6j5v, assembly 1, side view.
Disease resistance RPP13-like protein 4 in PDB entry 6j5v, assembly 1, top view.
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Probable serine/threonine-protein kinase PBL2
Chain: C
Length: 426 amino acids
Theoretical weight: 46.36 KDa
Source organism: Arabidopsis thaliana
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: O49839 (Residues: 1-426; Coverage: 100%)
Gene names: APK2A, At1g14370, F14L17.14, KIN1, PBL2
Pfam: Protein tyrosine and serine/threonine kinase
InterPro:
Probable serine/threonine-protein kinase PBL2 in PDB entry 6j5v, assembly 1, front view.
Probable serine/threonine-protein kinase PBL2 in PDB entry 6j5v, assembly 1, side view.
Probable serine/threonine-protein kinase PBL2 in PDB entry 6j5v, assembly 1, top view.
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Serine/threonine-protein kinase ZRK1
Chain: B
Length: 351 amino acids
Theoretical weight: 40.14 KDa
Source organism: Arabidopsis thaliana
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q9SVY5 (Residues: 1-351; Coverage: 100%)
Gene names: At3g57710, F15B8.100, QRX3, RKS1, ZRK1
Pfam: Protein kinase domain
InterPro:
Serine/threonine-protein kinase ZRK1 in PDB entry 6j5v, assembly 1, front view.
Serine/threonine-protein kinase ZRK1 in PDB entry 6j5v, assembly 1, side view.
Serine/threonine-protein kinase ZRK1 in PDB entry 6j5v, assembly 1, top view.
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