6m49

Electron Microscopy
3.7Å resolution

cryo-EM structure of Scap/Insig complex in the present of 25-hydroxyl cholesterol.

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-171390 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin-induced gene 2 protein Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 24.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9Y5U4 (Residues: 1-225; Coverage: 100%)
Gene name: INSIG2
Sequence domains: Insulin-induced protein (INSIG)
Sterol regulatory element-binding protein cleavage-activating protein Chain: B
Molecule details ›
Chain: B
Length: 572 amino acids
Theoretical weight: 63.3 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q12770 (Residues: 1-735; Coverage: 45%)
  • Best match: Q12770-4 (Residues: 1-343)
Gene names: KIAA0199, PSEC0227, SCAP
Sequence domains: Sterol-sensing domain of SREBP cleavage-activation

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-30074
Expression system: Homo sapiens