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Crystal structure of Streptococcus pyogenes endo-beta-N-acetylglucosaminidase (EndoS2) with high-mannose glycan

X-ray diffraction
2.5Å resolution
PDB entry 6mdv coloured by chain, front view.
PDB entry 6mdv coloured by chain, side view.
PDB entry 6mdv coloured by chain, top view.
Source organism: Streptococcus pyogenes
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Endo-beta-N-acetylglucosaminidase EndoS2
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Multimeric state: monomeric
Accessible surface area: 35143.66 Å2
Buried surface area: 2765.91 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-197486
Assembly 2
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Assembly Name: Endo-beta-N-acetylglucosaminidase EndoS2
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Multimeric state: monomeric
Accessible surface area: 35408.13 Å2
Buried surface area: 2361.92 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-197486

Macromolecules

Endo-beta-N-acetylglucosaminidase EndoS2
Chains: A, B
Length: 802 amino acids
Theoretical weight: 90.88 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: T1WGN1 (Residues: 44-843; Coverage: 99%)
Gene name: endoS2
Pfam: Endo-beta-N-acetylglucosaminidase F2, Ig-like domain
InterPro:
Endo-beta-N-acetylglucosaminidase EndoS2 in PDB entry 6mdv, assembly 1, front view.
Endo-beta-N-acetylglucosaminidase EndoS2 in PDB entry 6mdv, assembly 1, side view.
Endo-beta-N-acetylglucosaminidase EndoS2 in PDB entry 6mdv, assembly 1, top view.
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