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Structures and operating principles of the replisome.

Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W
Science 363 (2019)
Related entries: 6n7i, 6n7s, 6n7t, 6n7v, 6n7w, 6n9u, 6n9v, 6n9w, 6n9x

Cited: 77 times
EuropePMC logo PMID: 30679383

Abstract

Visualization in atomic detail of the replisome that performs concerted leading- and lagging-DNA strand synthesis at a replication fork has not been reported. Using bacteriophage T7 as a model system, we determined cryo-electron microscopy structures up to 3.2-angstroms resolution of helicase translocating along DNA and of helicase-polymerase-primase complexes engaging in synthesis of both DNA strands. Each domain of the spiral-shaped hexameric helicase translocates sequentially hand-over-hand along a single-stranded DNA coil, akin to the way AAA+ ATPases (adenosine triphosphatases) unfold peptides. Two lagging-strand polymerases are attached to the primase, ready for Okazaki fragment synthesis in tandem. A β hairpin from the leading-strand polymerase separates two parental DNA strands into a T-shaped fork, thus enabling the closely coupled helicase to advance perpendicular to the downstream DNA duplex. These structures reveal the molecular organization and operating principles of a replisome.

Reviews - 6n7n mentioned but not cited (1)

  1. Single-Molecule Insights Into the Dynamics of Replicative Helicases. Spinks RR, Spenkelink LM, Dixon NE, van Oijen AM. Front Mol Biosci 8 741718 (2021)

Articles - 6n7n mentioned but not cited (3)

  1. Structures and operating principles of the replisome. Gao Y, Cui Y, Fox T, Lin S, Wang H, de Val N, Zhou ZH, Yang W. Science 363 eaav7003 (2019)
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