6sb0

Electron Microscopy
5.5Å resolution

cryo-EM structure of mTORC1 bound to PRAS40-fused active RagA/C GTPases

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154634 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Serine/threonine-protein kinase mTOR Chains: A, B
Molecule details ›
Chains: A, B
Length: 2549 amino acids
Theoretical weight: 287.01 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P42345 (Residues: 60-2549; Coverage: 98%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
Target of rapamycin complex subunit LST8 Chains: E, H
Molecule details ›
Chains: E, H
Length: 326 amino acids
Theoretical weight: 35.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat
Ras-related GTP-binding protein A Chains: C, I
Ras-related GTP-binding protein C Chains: D, J
Regulatory-associated protein of mTOR Chains: N, Y
Proline-rich AKT1 substrate 1 Chains: O, T

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 5.5Å
Relevant EMDB volumes: EMD-10132
Expression systems:
  • Homo sapiens
  • Escherichia coli