6zkb

Electron Microscopy
2.9Å resolution

Membrane domain of closed complex I during turnover

Released:
Source organism: Ovis aries
Primary publication:
The coupling mechanism of mammalian respiratory complex I.
Science 370 (2020)
PMID: 32972993
Related structures: EMD-11243

Function and Biology Details

Reaction catalysed:
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 29-mer (preferred)
PDBe Complex ID:
PDB-CPX-131290 (preferred)
Entry contents:
29 distinct polypeptide molecules
Macromolecules (29 distinct):
Mitochondrial complex I, 49 kDa subunit Chain: 4
Molecule details ›
Chain: 4
Length: 457 amino acids
Theoretical weight: 52.02 KDa
Source organism: Ovis aries
NADH-ubiquinone oxidoreductase chain 5 Chain: L
Molecule details ›
Chain: L
Length: 606 amino acids
Theoretical weight: 68.44 KDa
Source organism: Ovis aries
NADH-ubiquinone oxidoreductase chain 4 Chain: M
NADH-ubiquinone oxidoreductase chain 2 Chain: N
Mitochondrial complex I, B14.7 subunit Chain: V
NADH:ubiquinone oxidoreductase subunit B5 Chain: W
Acyl carrier protein Chain: X
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Chain: Y
Mitochondrial complex I, PDSW subunit Chain: Z
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Chain: k
NADH:ubiquinone oxidoreductase subunit S5 Chain: l
NADH:ubiquinone oxidoreductase subunit A3 Chain: m
NADH:ubiquinone oxidoreductase subunit B3 Chain: n
NADH dehydrogenase [ubiquinone] 1 subunit C2 Chain: o
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Chain: p
Mitochondrial complex I, B16.6 subunit Chain: q
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 Chain: r
NADH:ubiquinone oxidoreductase subunit B7 Chain: s
NADH:ubiquinone oxidoreductase subunit B9 Chain: t
NADH:ubiquinone oxidoreductase subunit B2 Chain: u
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Chain: v
Mitochondrial complex I, ESSS subunit Chain: w
Mitochondrial complex I, KFYI subunit Chain: x
Mitochondrial complex I, MNLL subunit Chain: y
Mitochondrial complex I, MWFE subunit Chain: z
NADH-ubiquinone oxidoreductase chain 3 Chain: A
NADH-ubiquinone oxidoreductase chain 1 Chain: H
NADH-ubiquinone oxidoreductase chain 6 Chain: J
NADH-ubiquinone oxidoreductase chain 4L Chain: K

Ligands and Environments

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 2.9Å
Relevant EMDB volumes: EMD-11243