6zkf

Electron Microscopy
2.8Å resolution

Complex I during turnover, open3

Released:
Source organism: Ovis aries
Primary publication:
The coupling mechanism of mammalian respiratory complex I.
Science 370 (2020)
PMID: 32972993
Related structures: EMD-11247

Function and Biology Details

Reaction catalysed:
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero 45-mer (preferred)
PDBe Complex ID:
PDB-CPX-282766 (preferred)
Entry contents:
44 distinct polypeptide molecules
Macromolecules (44 distinct):
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: 1
Molecule details ›
Chain: 1
Length: 464 amino acids
Theoretical weight: 50.69 KDa
Source organism: Ovis aries
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Chain: 2
Molecule details ›
Chain: 2
Length: 246 amino acids
Theoretical weight: 27.37 KDa
Source organism: Ovis aries
NADH:ubiquinone oxidoreductase core subunit S1 Chain: 3
Mitochondrial complex I, 49 kDa subunit Chain: 4
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial Chain: 5
Mitochondrial complex I, PSST subunit Chain: 6
Mitochondrial complex I, TYKY subunit Chain: 9
NADH-ubiquinone oxidoreductase chain 3 Chain: A
NADH-ubiquinone oxidoreductase chain 1 Chain: H
NADH-ubiquinone oxidoreductase chain 6 Chain: J
NADH-ubiquinone oxidoreductase chain 4L Chain: K
NADH-ubiquinone oxidoreductase chain 5 Chain: L
NADH-ubiquinone oxidoreductase chain 4 Chain: M
NADH-ubiquinone oxidoreductase chain 2 Chain: N
Mitochondrial complex I, B14.7 subunit Chain: V
NADH:ubiquinone oxidoreductase subunit B5 Chain: W
Acyl carrier protein Chains: X, j
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 Chain: Y
Mitochondrial complex I, PDSW subunit Chain: Z
Mitochondrial complex I, 10 kDa subunit Chain: a
Mitochondrial complex I, 13 kDa subunit Chain: b
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: c
NADH:ubiquinone oxidoreductase subunit A9 Chain: d
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Chain: e
Mitochondrial complex I, B13 subunit Chain: f
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Chain: g
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 Chain: h
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Chain: i
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial Chain: k
NADH:ubiquinone oxidoreductase subunit S5 Chain: l
NADH:ubiquinone oxidoreductase subunit A3 Chain: m
NADH:ubiquinone oxidoreductase subunit B3 Chain: n
NADH dehydrogenase [ubiquinone] 1 subunit C2 Chain: o
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 Chain: p
Mitochondrial complex I, B16.6 subunit Chain: q
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 Chain: r
NADH:ubiquinone oxidoreductase subunit B7 Chain: s
NADH:ubiquinone oxidoreductase subunit B9 Chain: t
NADH:ubiquinone oxidoreductase subunit B2 Chain: u
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial Chain: v
Mitochondrial complex I, ESSS subunit Chain: w
Mitochondrial complex I, KFYI subunit Chain: x
Mitochondrial complex I, MNLL subunit Chain: y
Mitochondrial complex I, MWFE subunit Chain: z

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-11247