› 7aho

Electron Microscopy
4.18Å resolution

RUVBL1-RUVBL2 heterohexameric ring after binding of RNA helicase DHX34

Released:
DOI: 10.2210/pdb7aho/pdb
PDB entry 7aho coloured by chain, front view.
PDB entry 7aho coloured by chain, side view.
PDB entry 7aho coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM.
López-Perrote A, Hug N, González-Corpas A, Rodríguez CF, Serna M, García-Martín C, Boskovic J, Fernandez-Leiro R, Caceres JF, Llorca O
OpenAccess logo Elife 9 (2020)
PMID: 33205750
Related structures: EMD-11789

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195084 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RuvB-like 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 476 amino acids
Theoretical weight: 52.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y265 (Residues: 1-456; Coverage: 100%)
Gene names: INO80H, NMP238, RUVBL1, TIP49, TIP49A
Sequence domains:
RuvB-like 2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 481 amino acids
Theoretical weight: 53.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y230 (Residues: 1-463; Coverage: 100%)
Gene names: CGI-46, INO80J, RUVBL2, TIP48, TIP49B
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 3 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.18Å
Relevant EMDB volumes: EMD-11789
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Translation-coupled mRNA quality control mechanisms.
Monaghan et al. (2023)
5 other articles

1 mention without citation

Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM.
López-Perrote et al. (2020)