› 7ay1

Electron Microscopy
3.7Å resolution

Cryo-EM structure of USP1-UAF1 bound to mono-ubiquitinated FANCD2, and FANCI

Released:
DOI: 10.2210/pdb7ay1/pdb
PDB entry 7ay1 coloured by chain, front view.
PDB entry 7ay1 coloured by chain, side view.
PDB entry 7ay1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of FANCD2 deubiquitination by USP1-UAF1.
Rennie ML, Arkinson C, Chaugule VK, Toth R, Walden H
Nat Struct Mol Biol 28 356-364 (2021)
PMID: 33795880
Related structures: EMD-11934

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121090 (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (6 distinct):
Fanconi anemia group I protein Chain: A
Molecule details ›
Chain: A
Length: 1335 amino acids
Theoretical weight: 150.46 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NVI1 (Residues: 1-1328; Coverage: 100%)
Gene names: FANCI, KIAA1794
Sequence domains:
Fanconi anemia group D2 protein Chain: B
Molecule details ›
Chain: B
Length: 1455 amino acids
Theoretical weight: 164.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9BXW9 (Residues: 1-1451; Coverage: 100%)
Gene names: FACD, FANCD2
Sequence domains: Fanconi anaemia protein FancD2 nuclease
Ubiquitin Chain: C
Molecule details ›
Chain: C
Length: 80 amino acids
Theoretical weight: 8.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62987 (Residues: 1-76; Coverage: 59%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family
Ubiquitin carboxyl-terminal hydrolase 1 Chain: D
Molecule details ›
Chain: D
Length: 786 amino acids
Theoretical weight: 88.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O94782 (Residues: 1-785; Coverage: 100%)
Gene name: USP1
Sequence domains: Ubiquitin carboxyl-terminal hydrolase
WD repeat-containing protein 48 Chain: E
Molecule details ›
Chain: E
Length: 694 amino acids
Theoretical weight: 78.3 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8TAF3 (Residues: 1-677; Coverage: 100%)
Gene names: KIAA1449, UAF1, WDR48
Sequence domains:
DNA (61-MER) Chains: S, T
Molecule details ›
Chains: S, T
Length: 29 nucleotides
Theoretical weight: 5.18 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-11934
Expression system: Spodoptera frugiperda

Quick links

Citations

3 review citations

Deubiquitinases in cancer.
Dewson et al. (2023)
2 more

2 mentions without citation

Cryo-EM reveals a mechanism of USP1 inhibition through a cryptic binding site.
Rennie et al. (2022)
1 more