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The complex of DNA with the C-terminal domain of TYE7 from Saccharomyces cerevisiae.

X-ray diffraction
2.55Å resolution
PDB entry 7f2f coloured by chain, front view.
PDB entry 7f2f coloured by chain, side view.
PDB entry 7f2f coloured by chain, top view.
Source organisms:
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Transcription factor TYE7 and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 15772.35 Å2
Buried surface area: 7495.12 Å2
Dissociation area: 1,561.8 Å2
Dissociation energy (ΔGdiss): 28.58 kcal/mol
Dissociation entropy (TΔSdiss): 11.25 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-121274

Macromolecules

Transcription factor TYE7
Chains: A, B
Length: 136 amino acids
Theoretical weight: 15.86 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P33122 (Residues: 165-291; Coverage: 44%)
Gene names: O6233, SGC1, TYE7, YOR344C
Pfam: Helix-loop-helix DNA-binding domain
InterPro:
Transcription factor TYE7 in PDB entry 7f2f, assembly 1, front view.
Transcription factor TYE7 in PDB entry 7f2f, assembly 1, side view.
Transcription factor TYE7 in PDB entry 7f2f, assembly 1, top view.
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DNA chain E
Name: DNA (5'-D(*CP*AP*GP*AP*TP*CP*AP*TP*GP*TP*GP*TP*GP*CP*C)-3')
Representative chains: E
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
DNA (5'-D(*CP*AP*GP*AP*TP*CP*AP*TP*GP*TP*GP*TP*GP*CP*C)-3') in PDB entry 7f2f, assembly 1, front view.
DNA (5'-D(*CP*AP*GP*AP*TP*CP*AP*TP*GP*TP*GP*TP*GP*CP*C)-3') in PDB entry 7f2f, assembly 1, side view.
DNA (5'-D(*CP*AP*GP*AP*TP*CP*AP*TP*GP*TP*GP*TP*GP*CP*C)-3') in PDB entry 7f2f, assembly 1, top view.
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DNA chain H
Name: DNA (5'-D(*GP*GP*GP*CP*AP*CP*AP*CP*AP*TP*GP*AP*TP*CP*T)-3')
Representative chains: H
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
DNA (5'-D(*GP*GP*GP*CP*AP*CP*AP*CP*AP*TP*GP*AP*TP*CP*T)-3') in PDB entry 7f2f, assembly 1, front view.
DNA (5'-D(*GP*GP*GP*CP*AP*CP*AP*CP*AP*TP*GP*AP*TP*CP*T)-3') in PDB entry 7f2f, assembly 1, side view.
DNA (5'-D(*GP*GP*GP*CP*AP*CP*AP*CP*AP*TP*GP*AP*TP*CP*T)-3') in PDB entry 7f2f, assembly 1, top view.
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