› 7m30

Electron Microscopy
3.81Å resolution

Cryo-EM structure of the HCMV pentamer bound by antibodies 1-103, 1-32 and 2-25

Released:
DOI: 10.2210/pdb7m30/pdb
PDB entry 7m30 coloured by chain, front view.
PDB entry 7m30 coloured by chain, side view.
PDB entry 7m30 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for HCMV Pentamer recognition by neuropilin 2 and neutralizing antibodies.
Wrapp D, Ye X, Ku Z, Su H, Jones HG, Wang N, Mishra AK, Freed DC, Li F, Tang A, Li L, Jaijyan DK, Zhu H, Wang D, Fu TM, Zhang N, An Z, McLellan JS
OpenAccess logo Sci Adv 8 eabm2546 (2022)
PMID: 35275718
Related structures: EMD-23640

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-207854 (preferred)
Entry contents:
10 distinct polypeptide molecules
Macromolecules (10 distinct):
Envelope glycoprotein L Chain: B
Molecule details ›
Chain: B
Length: 248 amino acids
Theoretical weight: 27.66 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: P16832 (Residues: 31-278; Coverage: 100%)
Gene names: UL115, gL
Sequence domains: Cytomegalovirus glycoprotein L
Uncharacterized protein UL128 Chain: C
Molecule details ›
Chain: C
Length: 144 amino acids
Theoretical weight: 16.68 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: P16837 (Residues: 28-171; Coverage: 84%)
Gene name: UL128
Uncharacterized protein UL130 Chain: D
Molecule details ›
Chain: D
Length: 189 amino acids
Theoretical weight: 21.76 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: P16772 (Residues: 26-214; Coverage: 88%)
Gene name: UL130
Sequence domains: HCMV glycoprotein pUL130
Protein UL131A Chain: E
Molecule details ›
Chain: E
Length: 111 amino acids
Theoretical weight: 13.01 KDa
Source organism: Human betaherpesvirus 5
Expression system: Homo sapiens
UniProt:
  • Canonical: F5HET4 (Residues: 19-129; Coverage: 100%)
Gene name: UL131A
2-25 Fab Heavy Chain Chain: F
Molecule details ›
Chain: F
Length: 234 amino acids
Theoretical weight: 25.21 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
2-25 Fab Light Chain Chain: G
Molecule details ›
Chain: G
Length: 212 amino acids
Theoretical weight: 22.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
1-32 Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 241 amino acids
Theoretical weight: 26.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
1-32 Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.32 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
1-103 Fab Heavy Chain Chain: M
Molecule details ›
Chain: M
Length: 232 amino acids
Theoretical weight: 24.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
1-103 Fab Light Chain Chain: N
Molecule details ›
Chain: N
Length: 210 amino acids
Theoretical weight: 22.84 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.81Å
Relevant EMDB volumes: EMD-23640
Expression system: Homo sapiens

Quick links

Citations

2 review citations

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
1 more