7mn8

Electron Microscopy
3.45Å resolution

Structure of the HER2/HER3/NRG1b Heterodimer Extracellular Domain bound to Trastuzumab Fab

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209111 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (7 distinct):
Receptor tyrosine-protein kinase erbB-3 Chain: A
Molecule details ›
Chain: A
Length: 1066 amino acids
Theoretical weight: 117.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P21860 (Residues: 1-1021; Coverage: 76%)
  • Best match: P21860-3 (Residues: 1-330)
Gene names: ERBB3, HER3
Sequence domains:
Neuregulin-1 Chain: H
Molecule details ›
Chain: H
Length: 87 amino acids
Theoretical weight: 9.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02297 (Residues: 176-237; Coverage: 10%)
  • Best match: Q02297-8 (Residues: 176-230)
Gene names: GGF, HGL, HRGA, NDF, NRG1, SMDF
Sequence domains: EGF-like domain
Maltose/maltodextrin-binding periplasmic protein; Receptor tyrosine-protein kinase erbB-2 Chain: B
Molecule details ›
Chain: B
Length: 1455 amino acids
Theoretical weight: 160.56 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P04626 (Residues: 1-1029; Coverage: 82%)
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
Gene names: ERBB2, HER2, JW3994, MLN19, NEU, NGL, b4034, malE
Sequence domains:
Trastuzumab Fab Light Chain Chain: C
Molecule details ›
Chain: C
Length: 225 amino acids
Theoretical weight: 24.66 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Trastuzumab Fab Heavy Chain Chain: D
Molecule details ›
Chain: D
Length: 237 amino acids
Theoretical weight: 25.36 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.45Å
Relevant EMDB volumes: EMD-23918
Expression systems:
  • Homo sapiens
  • Escherichia coli