› 7opw

X-ray diffraction
1.81Å resolution

Ternary complex of 14-3-3 sigma, Estrogen Receptor alfa phosphopeptide, and WQ136

Released:
DOI: 10.2210/pdb7opw/pdb
PDB entry 7opw coloured by chain, front view.
PDB entry 7opw coloured by chain, side view.
PDB entry 7opw coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Centorrino F, Ottmann C

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136921 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31947 (Residues: 1-248; Coverage: 100%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Estrogen receptor Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.73 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P03372 (Residues: 581-595; Coverage: 3%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Oestrogen-type nuclear receptor final C-terminal

Ligands and Environments

4 bound ligands:
Ligand 0AW 3 x 0AW
Ligand MG 2 x MG
Ligand CL 1 x CL
Ligand BME 1 x BME
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: C2221
Unit cell:
a: 81.981Å b: 110.495Å c: 61.228Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.229
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links