› 7qhs

Electron Microscopy
3.3Å resolution

S. cerevisiae CMGE nucleating origin DNA melting

Released:
DOI: 10.2210/pdb7qhs/pdb
PDB entry 7qhs coloured by chain, front view.
PDB entry 7qhs coloured by chain, side view.
PDB entry 7qhs coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Mechanism of replication origin melting nucleated by CMG helicase assembly.
Lewis JS, Gross MH, Sousa J, Henrikus SS, Greiwe JF, Nans A, Diffley JFX, Costa A
Nature (2022)
PMID: 35705812
Related structures: EMD-13978

Function and Biology Details

Reactions catalysed: 
ATP + H(2)O = ADP + phosphate
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
PDBe Complex ID:
PDB-CPX-105545 (preferred)
Entry contents:
13 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (15 distinct):
DNA replication licensing factor MCM2 Chain: 2
Molecule details ›
Chain: 2
Length: 868 amino acids
Theoretical weight: 98.91 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5Q1S9 (Residues: 1-868; Coverage: 100%)
Gene names: GI527_G0000178, MCM2
Sequence domains:
DNA replication licensing factor MCM3 Chain: 3
Molecule details ›
Chain: 3
Length: 1006 amino acids
Theoretical weight: 111.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24279 (Residues: 1-971; Coverage: 100%)
Gene names: MCM3, SYGP-ORF23, YEL032W
Sequence domains:
DNA replication licensing factor MCM4 Chain: 4
Molecule details ›
Chain: 4
Length: 933 amino acids
Theoretical weight: 105.14 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P30665 (Residues: 1-933; Coverage: 100%)
Gene names: CDC54, HCD21, MCM4, YP9531.13, YPR019W
Sequence domains:
DNA replication licensing factor MCM6 Chain: 6
Molecule details ›
Chain: 6
Length: 1017 amino acids
Theoretical weight: 113.11 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53091 (Residues: 1-1017; Coverage: 100%)
Gene names: MCM6, YGL201C
Sequence domains:
DNA replication licensing factor MCM7 Chain: 7
Molecule details ›
Chain: 7
Length: 845 amino acids
Theoretical weight: 95.05 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38132 (Residues: 1-845; Coverage: 100%)
Gene names: CDC47, MCM7, YBR1441, YBR202W
Sequence domains:
DNA replication complex GINS protein PSF1 Chain: H
Molecule details ›
Chain: H
Length: 208 amino acids
Theoretical weight: 24.23 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6A5Q203 (Residues: 1-208; Coverage: 100%)
Gene names: GI527_G0000936, PSF1
Sequence domains: GINS complex protein helical bundle domain
DNA replication complex GINS protein PSF2 Chain: I
Molecule details ›
Chain: I
Length: 213 amino acids
Theoretical weight: 25.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6A5PX40 (Residues: 1-213; Coverage: 100%)
Gene names: GI527_G0003179, PSF2
Sequence domains: GINS complex protein helical bundle domain
DNA replication complex GINS protein PSF3 Chain: C
Molecule details ›
Chain: C
Length: 229 amino acids
Theoretical weight: 25.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12146 (Residues: 1-194; Coverage: 100%)
Gene names: PSF3, YOL146W
Sequence domains:
DNA replication complex GINS protein SLD5 Chain: D
Molecule details ›
Chain: D
Length: 294 amino acids
Theoretical weight: 33.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03406 (Residues: 1-294; Coverage: 100%)
Gene names: SLD5, YDR489W
Sequence domains:
Cell division control protein 45 Chain: E
Molecule details ›
Chain: E
Length: 657 amino acids
Theoretical weight: 75.15 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q08032 (Residues: 1-203, 204-650; Coverage: 100%)
Gene names: CDC45, L8004.11, SLD4, YLR103C
Sequence domains: CDC45-like protein
DNA polymerase epsilon subunit B Chain: F
Molecule details ›
Chain: F
Length: 689 amino acids
Theoretical weight: 78.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24482 (Residues: 1-689; Coverage: 100%)
Gene names: DPB2, P9705.7, YPR175W
Sequence domains: DNA polymerase alpha/epsilon subunit B
DNA polymerase epsilon catalytic subunit A Chain: G
Molecule details ›
Chain: G
Length: 2222 amino acids
Theoretical weight: 255.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P21951 (Residues: 1-2222; Coverage: 100%)
Gene names: DUN2, N0825, POL2, YNL262W
Sequence domains:
DNA replication licensing factor MCM5 Chain: 5
Molecule details ›
Chain: 5
Length: 775 amino acids
Theoretical weight: 86.51 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: A0A6A5PUY8 (Residues: 1-775; Coverage: 100%)
Gene names: GI527_G0004088, MCM5
Sequence domains:
DNA (26-MER) Chain: A
Molecule details ›
Chain: A
Length: 26 nucleotides
Theoretical weight: 8.1 KDa
DNA (26-MER) Chain: B
Molecule details ›
Chain: B
Length: 26 nucleotides
Theoretical weight: 7.86 KDa

Ligands and Environments

4 bound ligands:
Ligand ATP 4 x ATP
Ligand ZN 7 x ZN
Ligand MG 3 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-13978
Expression systems:
  • Saccharomyces cerevisiae
  • Escherichia coli

Quick links

Citations

7 review citations

A Journey to the Core of the Plant Cell Cycle.
Gutierrez C. (2022)
6 more