7qqv

X-ray diffraction
2.25Å resolution

SpCas9 bound to FANCF off-target3 DNA substrate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-511958 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: B
Molecule details ›
Chain: B
Length: 1368 amino acids
Theoretical weight: 158.59 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
FANCF off-target3 target strand Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.66 KDa
Source organism: synthetic construct
Expression system: Not provided
FANCF off-target3 non-target strand Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: synthetic construct
Expression system: Not provided
FANCF sgRNA Chain: A
Molecule details ›
Chain: A
Length: 85 nucleotides
Theoretical weight: 27.41 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 179.019Å b: 67.145Å c: 187.767Å
α: 90° β: 111.141° γ: 90°
R-values:
R R work R free
0.196 0.195 0.23
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided