Assemblies
Assembly Name:
CRISPR-associated endonuclease Cas9/Csn1 and RNA and DNA
Multimeric state:
hetero tetramer
Accessible surface area:
76030.89 Å2
Buried surface area:
22611.13 Å2
Dissociation area:
945.17
Å2
Dissociation energy (ΔGdiss):
12.03
kcal/mol
Dissociation entropy (TΔSdiss):
9.88
kcal/mol
Symmetry number:
1
PDBe Complex ID:
PDB-CPX-511958
Macromolecules
Chain: B
Length: 1368 amino acids
Theoretical weight: 158.59 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
Pfam:
Length: 1368 amino acids
Theoretical weight: 158.59 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Pfam:
- Bridge helix of CRISPR-associated endonuclease Cas9
- REC lobe of CRISPR-associated endonuclease Cas9
- HNH endonuclease
- Cas9 RuvC domain
- PAM-interacting domain of CRISPR-associated endonuclease Cas9
Name:
FANCF off-target3 target strand
Representative chains: C
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 28 nucleotides
Theoretical weight: 8.66 KDa
Representative chains: C
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 28 nucleotides
Theoretical weight: 8.66 KDa
Name:
FANCF off-target3 non-target strand
Representative chains: D
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Representative chains: D
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Name:
FANCF sgRNA
Representative chains: A
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 85 nucleotides
Theoretical weight: 27.41 KDa
Representative chains: A
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 85 nucleotides
Theoretical weight: 27.41 KDa
