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T.thermophilus 30S ribosome with KsgA, class K2

Electron Microscopy
3.6Å resolution
PDB entry 7v2n coloured by chain, front view.
PDB entry 7v2n coloured by chain, side view.
PDB entry 7v2n coloured by chain, top view.
Source organisms:
Assembly composition:
hetero 22-mer (preferred)
Entry contents: 21 distinct polypeptide molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 22-mer
Accessible surface area: 280129.32 Å2
Buried surface area: 90651.19 Å2
Dissociation area: 1,517.58 Å2
Dissociation energy (ΔGdiss): 13.71 kcal/mol
Dissociation entropy (TΔSdiss): 14.7 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-250071

Macromolecules

Small ribosomal subunit protein uS2
Chain: B
Length: 256 amino acids
Theoretical weight: 29.32 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80371 (Residues: 1-256; Coverage: 100%)
Gene names: TTHA0861, rps2, rpsB
Pfam: Ribosomal protein S2
InterPro:
Small ribosomal subunit protein uS2 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS2 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS2 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS3
Chain: C
Length: 239 amino acids
Theoretical weight: 26.75 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80372 (Residues: 1-239; Coverage: 100%)
Gene names: TTHA1686, rps3, rpsC
Pfam:
InterPro:
Small ribosomal subunit protein uS3 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS3 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS3 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS4
Chain: D
Length: 209 amino acids
Theoretical weight: 24.37 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80373 (Residues: 1-209; Coverage: 100%)
Gene names: TTHA1665, rps4, rpsD
Pfam:
InterPro:
Small ribosomal subunit protein uS4 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS4 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS4 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS5
Chain: E
Length: 162 amino acids
Theoretical weight: 17.58 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHQ5 (Residues: 1-162; Coverage: 100%)
Gene names: TTHA1675, rpsE
Pfam:
InterPro:
Small ribosomal subunit protein uS5 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS5 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS5 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein bS6
Chain: F
Length: 101 amino acids
Theoretical weight: 11.99 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLP8 (Residues: 1-101; Coverage: 100%)
Gene names: TTHA0245, rpsF
Pfam: Ribosomal protein S6
InterPro:
Small ribosomal subunit protein bS6 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein bS6 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein bS6 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS7
Chain: G
Length: 156 amino acids
Theoretical weight: 18.05 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P17291 (Residues: 1-156; Coverage: 100%)
Gene names: TTHA1696, rps7, rpsG
Pfam: Ribosomal protein S7p/S5e
InterPro:
Small ribosomal subunit protein uS7 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS7 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS7 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS8
Chain: H
Length: 138 amino acids
Theoretical weight: 15.87 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY9 (Residues: 1-138; Coverage: 100%)
Gene names: TTHA1678, rpsH
Pfam: Ribosomal protein S8
InterPro:
Small ribosomal subunit protein uS8 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS8 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS8 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS9
Chain: I
Length: 128 amino acids
Theoretical weight: 14.41 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80374 (Residues: 1-128; Coverage: 100%)
Gene names: TTHA1464, rps9, rpsI
Pfam: Ribosomal protein S9/S16
InterPro:
Small ribosomal subunit protein uS9 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS9 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS9 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS10
Chain: J
Length: 105 amino acids
Theoretical weight: 11.95 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN7 (Residues: 1-105; Coverage: 100%)
Gene names: TTHA1693, rpsJ
Pfam: Ribosomal protein S10p/S20e
InterPro:
Small ribosomal subunit protein uS10 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS10 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS10 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS11
Chain: K
Length: 129 amino acids
Theoretical weight: 13.74 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80376 (Residues: 1-129; Coverage: 100%)
Gene names: TTHA1666, rps11, rpsK
Pfam: Ribosomal protein S11
InterPro:
Small ribosomal subunit protein uS11 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS11 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS11 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS12
Chain: L
Length: 132 amino acids
Theoretical weight: 14.64 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHN3 (Residues: 1-132; Coverage: 100%)
Gene names: TTHA1697, rpsL
Pfam: Ribosomal protein S12/S23
InterPro:
Small ribosomal subunit protein uS12 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS12 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS12 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS13
Chain: M
Length: 126 amino acids
Theoretical weight: 14.34 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80377 (Residues: 1-126; Coverage: 100%)
Gene names: TTHA1667, rps13, rpsM
Pfam: Ribosomal protein S13/S18
InterPro:
Small ribosomal subunit protein uS13 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS13 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS13 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS14
Chain: N
Length: 61 amino acids
Theoretical weight: 7.16 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY6 (Residues: 1-61; Coverage: 100%)
Gene names: TTHA1679, rpsN, rpsZ
Pfam: Ribosomal protein S14p/S29e
InterPro:
Small ribosomal subunit protein uS14 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS14 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS14 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS15
Chain: O
Length: 89 amino acids
Theoretical weight: 10.58 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJ76 (Residues: 1-89; Coverage: 100%)
Gene names: TTHA1138, rpsO
Pfam: Ribosomal protein S15
InterPro:
Small ribosomal subunit protein uS15 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS15 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS15 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein bS16
Chain: P
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SJH3 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA1035, rpsP
Pfam: Ribosomal protein S16
InterPro:
Small ribosomal subunit protein bS16 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein bS16 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein bS16 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS17
Chain: Q
Length: 105 amino acids
Theoretical weight: 12.33 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P0DOY7 (Residues: 1-105; Coverage: 100%)
Gene names: TTHA1683, rpsQ
Pfam: Ribosomal protein S17
InterPro:
Small ribosomal subunit protein uS17 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS17 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS17 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein bS18
Chain: R
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SLQ0 (Residues: 1-88; Coverage: 100%)
Gene names: TTHA0243, rpsR
Pfam: Ribosomal protein S18
InterPro:
Small ribosomal subunit protein bS18 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein bS18 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein bS18 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein uS19
Chain: S
Length: 93 amino acids
Theoretical weight: 10.61 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHP2 (Residues: 1-93; Coverage: 100%)
Gene names: TTHA1688, rpsS
Pfam: Ribosomal protein S19
InterPro:
Small ribosomal subunit protein uS19 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein uS19 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein uS19 in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein bS20
Chain: T
Length: 106 amino acids
Theoretical weight: 11.74 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80380 (Residues: 1-106; Coverage: 100%)
Gene names: TTHA1397, rps20, rpsT
Pfam: Ribosomal protein S20
InterPro:
Small ribosomal subunit protein bS20 in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein bS20 in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein bS20 in PDB entry 7v2n, assembly 1, top view.
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Ribosomal RNA small subunit methyltransferase A
Chain: U
Length: 298 amino acids
Theoretical weight: 33.59 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37468 (Residues: 1-292; Coverage: 100%)
Gene names: BSU00420, ksgA, rsmA
Pfam: Ribosomal RNA adenine dimethylase
InterPro:
Ribosomal RNA small subunit methyltransferase A in PDB entry 7v2n, assembly 1, front view.
Ribosomal RNA small subunit methyltransferase A in PDB entry 7v2n, assembly 1, side view.
Ribosomal RNA small subunit methyltransferase A in PDB entry 7v2n, assembly 1, top view.
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Small ribosomal subunit protein bTHX
Chain: V
Length: 27 amino acids
Theoretical weight: 3.35 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SIH3 (Residues: 1-27; Coverage: 100%)
Gene names: TTHA1396, rpsU
Pfam: 30S ribosomal protein Thx
InterPro:
Small ribosomal subunit protein bTHX in PDB entry 7v2n, assembly 1, front view.
Small ribosomal subunit protein bTHX in PDB entry 7v2n, assembly 1, side view.
Small ribosomal subunit protein bTHX in PDB entry 7v2n, assembly 1, top view.
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RNA chain A
Name: 16S ribosomal RNA
Representative chains: A
Length: 1522 nucleotides
Theoretical weight: 493.96 KDa
Rfam: Bacterial small subunit ribosomal RNA
16S ribosomal RNA in PDB entry 7v2n, assembly 1, front view.
16S ribosomal RNA in PDB entry 7v2n, assembly 1, side view.
16S ribosomal RNA in PDB entry 7v2n, assembly 1, top view.
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