7vwj

Electron Microscopy
2.6Å resolution

Matrix arm of deactive state CI from rotenone-NADH dataset

Released:
Source organism: Sus scrofa
Primary publication:
The coupling mechanism of mammalian mitochondrial complex I.
Nat Struct Mol Biol 29 172-182 (2022)
PMID: 35145322
Related structures: EMD-32154

Function and Biology Details

Reaction catalysed:
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-496410 (preferred)
Entry contents:
18 distinct polypeptide molecules
Macromolecules (18 distinct):
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 47.27 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 176 amino acids
Theoretical weight: 20.21 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial Chain: C
Molecule details ›
Chain: C
Length: 156 amino acids
Theoretical weight: 17.87 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 Chain: E
Molecule details ›
Chain: E
Length: 115 amino acids
Theoretical weight: 13.81 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 Chain: F
Molecule details ›
Chain: F
Length: 86 amino acids
Theoretical weight: 9.84 KDa
Source organism: Sus scrofa
Acyl carrier protein Chain: G
Molecule details ›
Chain: G
Length: 88 amino acids
Theoretical weight: 10.13 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 Chain: H
Molecule details ›
Chain: H
Length: 112 amino acids
Theoretical weight: 12.95 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 Chain: I
Molecule details ›
Chain: I
Length: 112 amino acids
Theoretical weight: 12.52 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial Chain: J
Molecule details ›
Chain: J
Length: 341 amino acids
Theoretical weight: 38.84 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial Chain: K
Molecule details ›
Chain: K
Length: 42 amino acids
Theoretical weight: 5.05 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial Chain: L
Molecule details ›
Chain: L
Length: 125 amino acids
Theoretical weight: 14.44 KDa
Source organism: Sus scrofa
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial Chain: M
Molecule details ›
Chain: M
Length: 690 amino acids
Theoretical weight: 75.77 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 Chain: N
Molecule details ›
Chain: N
Length: 144 amino acids
Theoretical weight: 17.03 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial Chain: O
Molecule details ›
Chain: O
Length: 217 amino acids
Theoretical weight: 23.83 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial Chain: P
Molecule details ›
Chain: P
Length: 208 amino acids
Theoretical weight: 24.52 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial Chain: Q
Molecule details ›
Chain: Q
Length: 385 amino acids
Theoretical weight: 44.04 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial Chain: T
Molecule details ›
Chain: T
Length: 96 amino acids
Theoretical weight: 10.57 KDa
Source organism: Sus scrofa
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 Chain: W
Molecule details ›
Chain: W
Length: 29 amino acids
Theoretical weight: 3.2 KDa
Source organism: Sus scrofa

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-32154