Structure analysis

Matrix arm of deactive state CI from rotenone-NADH dataset

Electron Microscopy
2.6Å resolution
Source organism: Sus scrofa
Assembly composition:
hetero octadecamer (preferred)
Entry contents: 18 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero octadecamer
Accessible surface area: 117764.18 Å2
Buried surface area: 98467.05 Å2
Dissociation area: 1,150.17 Å2
Dissociation energy (ΔGdiss): 0.83 kcal/mol
Dissociation entropy (TΔSdiss): 12.22 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-236689

Macromolecules

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Chain: B
Length: 176 amino acids
Theoretical weight: 20.21 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287BDC0 (Residues: 37-212; Coverage: 83%)
Gene name: NDUFS8
Pfam: 4Fe-4S dicluster domain
InterPro:

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Chain: C
Length: 156 amino acids
Theoretical weight: 17.87 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1TL60 (Residues: 41-196; Coverage: 80%)
Gene name: NDUFS7
Pfam: NADH ubiquinone oxidoreductase, 20 Kd subunit
InterPro:

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Chain: E
Length: 115 amino acids
Theoretical weight: 13.81 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SJP6 (Residues: 40-154; Coverage: 75%)
Gene name: NDUFA6
Pfam: Complex 1 protein (LYR family)
InterPro:

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Chain: F
Length: 86 amino acids
Theoretical weight: 9.84 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1RGE3 (Residues: 14-99; Coverage: 87%)
Gene name: NDUFA2
Pfam: Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain
InterPro:

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Chain: G
Length: 88 amino acids
Theoretical weight: 10.13 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AAR5 (Residues: 69-156; Coverage: 56%)
Gene name: NDUFAB1
Pfam: Phosphopantetheine attachment site
InterPro:

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Chain: H
Length: 112 amino acids
Theoretical weight: 12.95 KDa
Source organism: Sus scrofa
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

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Chain: I
Length: 112 amino acids
Theoretical weight: 12.52 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1VLA2 (Residues: 2-113; Coverage: 99%)
Gene name: NDUFA7
Pfam: NADH:ubiquinone oxidoreductase subunit B14.5a (Complex I-B14.5a)
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7

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Chain: J
Length: 341 amino acids
Theoretical weight: 38.84 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287AE88 (Residues: 36-406; Coverage: 84%)
Gene name: NDUFA9
Pfam: NAD dependent epimerase/dehydratase family
InterPro:

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Chain: K
Length: 42 amino acids
Theoretical weight: 5.05 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: I3LRR4 (Residues: 69-110; Coverage: 38%)
Gene name: NDUFV3
Pfam: NADH dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial
InterPro: NADH-ubiquinone oxidoreductase flavoprotein 3

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Chain: L
Length: 125 amino acids
Theoretical weight: 14.44 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A4X1SN37 (Residues: 51-175; Coverage: 71%)
Gene name: NDUFS4
Pfam: NADH dehydrogenase ubiquinone Fe-S protein 4
InterPro:

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Chain: N
Length: 144 amino acids
Theoretical weight: 17.03 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1SQP4 (Residues: 2-145; Coverage: 99%)
Gene name: NDUFA12
Pfam: NADH ubiquinone oxidoreductase subunit NDUFA12
InterPro: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

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Chain: O
Length: 217 amino acids
Theoretical weight: 23.83 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A287BG40 (Residues: 33-249; Coverage: 87%)
Gene name: NDUFV2
Pfam: Thioredoxin-like [2Fe-2S] ferredoxin
InterPro:

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Chain: T
Length: 96 amino acids
Theoretical weight: 10.57 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: F1S031 (Residues: 28-123; Coverage: 78%)
Gene name: NDUFS6
Pfam: Zinc-finger domain
InterPro:

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Chain: W
Length: 29 amino acids
Theoretical weight: 3.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: A0A8D1NRT6 (Residues: 3-31; Coverage: 20%)
Gene name: NDUFA13
InterPro: GRIM-19

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