› 7y7z

Electron Microscopy
3.2Å resolution

Cryo-EM structure of human GABA transporter GAT1 bound with tiagabine in NaCl solution in an inward-open state at 3.2 angstrom

Released:
DOI: 10.2210/pdb7y7z/pdb
PDB entry 7y7z coloured by chain, front view.
PDB entry 7y7z coloured by chain, side view.
PDB entry 7y7z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for substrate recognition and transport of human GABA transporter GAT1.
Zhu A, Huang J, Kong F, Tan J, Lei J, Yuan Y, Yan C
Nat Struct Mol Biol 30 1012-1022 (2023)
PMID: 37400655
Related structures: EMD-33675

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151757 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sodium- and chloride-dependent GABA transporter 1 Chain: A
Molecule details ›
Chain: A
Length: 632 amino acids
Theoretical weight: 70.9 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P30531 (Residues: 1-599; Coverage: 100%)
Gene names: GABATR, GABT1, GAT1, SLC6A1
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

3 bound ligands:
Ligand TGI 1 x TGI
Ligand NAG 3 x NAG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-33675
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

Probing binding and occlusion of substrate in the human creatine transporter-1 by computation and mutagenesis.
Clarke et al. (2024)