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Conformational changes in mitochondrial complex I of the thermophilic eukaryote Chaetomium thermophilum.
<div class='caption-title'>CryoEM density map and modeled structure of mitochondrial complex I from C. thermophilum.</div>
<div class='caption-title'>Cofactors and lipids in C. thermophilum complex I.</div>
Laube E, Meier-Credo J, Langer JD, Kühlbrandt W
OpenAccess logo Sci Adv 8 eadc9952 (2022)
Related entries: 7zm7, 7zm8, 7zmb, 7zme, 7zmh

Cited: 8 times
EuropePMC logo PMID: 36427319

Abstract

Mitochondrial complex I is a redox-driven proton pump that generates proton-motive force across the inner mitochondrial membrane, powering oxidative phosphorylation and ATP synthesis in eukaryotes. We report the structure of complex I from the thermophilic fungus Chaetomium thermophilum, determined by cryoEM up to 2.4-Å resolution. We show that the complex undergoes a transition between two conformations, which we refer to as state 1 and state 2. The conformational switch is manifest in a twisting movement of the peripheral arm relative to the membrane arm, but most notably in substantial rearrangements of the Q-binding cavity and the E-channel, resulting in a continuous aqueous passage from the E-channel to subunit ND5 at the far end of the membrane arm. The conformational changes in the complex interior resemble those reported for mammalian complex I, suggesting a highly conserved, universal mechanism of coupling electron transport to proton pumping.

Articles - 7zmg mentioned but not cited (1)

  1. Resting mitochondrial complex I from Drosophila melanogaster adopts a helix-locked state. Padavannil A, Murari A, Rhooms SK, Owusu-Ansah E, Letts JA. Elife 12 e84415 (2023)


Reviews citing this publication (3)

  1. The biogenesis and regulation of the plant oxidative phosphorylation system. Ghifari AS, Saha S, Murcha MW. Plant Physiol 192 728-747 (2023)
  2. From the 'black box' to 'domino effect' mechanism: what have we learned from the structures of respiratory complex I. Sazanov LA. Biochem J 480 319-333 (2023)
  3. Mitochondrial complex I ROS production and redox signaling in hypoxia. Okoye CN, Koren SA, Wojtovich AP. Redox Biol 67 102926 (2023)

Articles citing this publication (4)

  1. Cryo-EM structure of the respiratory I + III2 supercomplex from Arabidopsis thaliana at 2 Å resolution. Klusch N, Dreimann M, Senkler J, Rugen N, Kühlbrandt W, Braun HP. Nat Plants 9 142-156 (2023)
  2. Plant-specific features of respiratory supercomplex I + III2 from Vigna radiata. Maldonado M, Fan Z, Abe KM, Letts JA. Nat Plants 9 157-168 (2023)
  3. Quinone Catalysis Modulates Proton Transfer Reactions in the Membrane Domain of Respiratory Complex I. Kim H, Saura P, Pöverlein MC, Gamiz-Hernandez AP, Kaila VRI. J Am Chem Soc 145 17075-17086 (2023)
  4. Investigation of hydrated channels and proton pathways in a high-resolution cryo-EM structure of mammalian complex I. Grba DN, Chung I, Bridges HR, Agip AA, Hirst J. Sci Adv 9 eadi1359 (2023)


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