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Structure of the peroxisomal Pex1/Pex6 ATPase complex bound to a substrate in twin seam state

Electron Microscopy
4.7Å resolution
PDB entry 8c0w coloured by chain, front view.
PDB entry 8c0w coloured by chain, side view.
PDB entry 8c0w coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero heptamer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero heptamer
Accessible surface area: 270777.98 Å2
Buried surface area: 51150.59 Å2
Dissociation area: 635.05 Å2
Dissociation energy (ΔGdiss): 4.83 kcal/mol
Dissociation entropy (TΔSdiss): 6.42 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-273419

Macromolecules

Peroxisomal ATPase PEX6
Chains: A, C, E
Length: 1030 amino acids
Theoretical weight: 115.69 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33760 (Residues: 1-1030; Coverage: 100%)
Gene names: N0310, PAS8, PEX6, YNL329C
Pfam:
Peroxisomal ATPase PEX6 in PDB entry 8c0w, assembly 1, front view.
Peroxisomal ATPase PEX6 in PDB entry 8c0w, assembly 1, side view.
Peroxisomal ATPase PEX6 in PDB entry 8c0w, assembly 1, top view.
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Peroxisomal ATPase PEX1
Chains: B, D, F
Length: 823 amino acids
Theoretical weight: 92.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P24004 (Residues: 201-1023; Coverage: 79%)
Gene names: PAS1, PEX1, YKL197C
Pfam:
Peroxisomal ATPase PEX1 in PDB entry 8c0w, assembly 1, front view.
Peroxisomal ATPase PEX1 in PDB entry 8c0w, assembly 1, side view.
Peroxisomal ATPase PEX1 in PDB entry 8c0w, assembly 1, top view.
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unknown peptide
Chain: R
Length: 9 amino acids
Theoretical weight: 784 Da
Source organism: Saccharomyces cerevisiae
unknown peptide in PDB entry 8c0w, assembly 1, front view.
unknown peptide in PDB entry 8c0w, assembly 1, side view.
unknown peptide in PDB entry 8c0w, assembly 1, top view.
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