› 8dy1

X-ray diffraction
2.68Å resolution

Crystal Structure of scFv CAT2200 LH in complex with IL-17A

Released:
DOI: 10.2210/pdb8dy1/pdb
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PDB entry 8dy1 coloured by chain, front view.
PDB entry 8dy1 coloured by chain, side view.
PDB entry 8dy1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
"Stapling" scFv for multispecific biotherapeutics of superior properties.
Boucher LE, Prinslow EG, Feldkamp M, Yi F, Nanjunda R, Wu SJ, Liu T, Lacy ER, Jacobs S, Kozlyuk N, Del Rosario B, Wu B, Aquino P, Davidson RC, Heyne S, Mazzanti N, Testa J, Diem MD, Gorre E, Mahan A, Nanda H, Gunawardena HP, Gervais A, Armstrong AA, Teplyakov A, Huang C, Zwolak A, Chowdhury P, Cheung WC, Luo J
OpenAccess logo MAbs 15 2195517 (2023)
PMID: 37074212

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-280174 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Interleukin-17A Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 14.21 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q16552 (Residues: 35-155; Coverage: 92%)
Gene names: CTLA8, IL17, IL17A
Sequence domains: Interleukin-17
scFv CAT2200 LH Chains: C, D
Molecule details ›
Chains: C, D
Length: 255 amino acids
Theoretical weight: 26.62 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 51.93Å b: 62.05Å c: 111.71Å
α: 90° β: 99.69° γ: 90°
R-values:
R R work R free
0.223 0.221 0.268
Expression system: Homo sapiens

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Citations

3 review citations

Structural modeling of antibody variable regions using deep learning-progress and perspectives on drug discovery.
Jaszczyszyn et al. (2023)
2 more

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