› › Structure analysis

TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene

Electron Microscopy
3.8Å resolution
PDB entry 8ffz coloured by chain, front view.
PDB entry 8ffz coloured by chain, side view.
PDB entry 8ffz coloured by chain, top view.
Source organisms:
Assembly composition:
hetero decamer (preferred)
Entry contents: 8 distinct polypeptide molecules
2 distinct DNA molecules

Quick links

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Multimeric state: hetero decamer
Accessible surface area: 237215.1 Å2
Buried surface area: 79905.97 Å2
Dissociation area: 1,672.19 Å2
Dissociation energy (ΔGdiss): 5.84 kcal/mol
Dissociation entropy (TΔSdiss): 15.24 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-218480

Macromolecules

Transcription factor IIIA
Chain: A
Length: 451 amino acids
Theoretical weight: 52.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P39933 (Residues: 1-429; Coverage: 100%)
Gene names: P9677.9, PZF1, TFC2, TFIIIA, YPR186C
Pfam:
Transcription factor IIIA in PDB entry 8ffz, assembly 1, front view.
Transcription factor IIIA in PDB entry 8ffz, assembly 1, side view.
Transcription factor IIIA in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar proteins

Transcription factor tau 138 kDa subunit
Chain: B
Length: 1160 amino acids
Theoretical weight: 132.31 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P34111 (Residues: 1-1160; Coverage: 100%)
Gene names: FUN24, TFC3, TSV115, YAL001C
Pfam:
Transcription factor tau 138 kDa subunit in PDB entry 8ffz, assembly 1, front view.
Transcription factor tau 138 kDa subunit in PDB entry 8ffz, assembly 1, side view.
Transcription factor tau 138 kDa subunit in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar proteins

Transcription factor tau 131 kDa subunit
Chain: C
Length: 1025 amino acids
Theoretical weight: 120.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33339 (Residues: 1-1025; Coverage: 100%)
Gene names: PCF1, TFC4, YGR047C
Pfam:
Transcription factor tau 131 kDa subunit in PDB entry 8ffz, assembly 1, front view.
Transcription factor tau 131 kDa subunit in PDB entry 8ffz, assembly 1, side view.
Transcription factor tau 131 kDa subunit in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar proteins

Transcription factor tau 95 kDa subunit
Chain: D
Length: 649 amino acids
Theoretical weight: 73.65 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32367 (Residues: 1-649; Coverage: 100%)
Gene names: TFC1, YBR0919, YBR123C
Pfam:
Transcription factor tau 95 kDa subunit in PDB entry 8ffz, assembly 1, front view.
Transcription factor tau 95 kDa subunit in PDB entry 8ffz, assembly 1, side view.
Transcription factor tau 95 kDa subunit in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar proteins

Transcription factor tau 91 kDa subunit
Chain: E
Length: 672 amino acids
Theoretical weight: 74.8 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q06339 (Residues: 1-672; Coverage: 100%)
Gene names: TFC6, YDR362C
Transcription factor tau 91 kDa subunit in PDB entry 8ffz, assembly 1, front view.
Transcription factor tau 91 kDa subunit in PDB entry 8ffz, assembly 1, side view.
Transcription factor tau 91 kDa subunit in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar proteins

Transcription factor tau 60 kDa subunit
Chain: F
Length: 588 amino acids
Theoretical weight: 67.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12308 (Residues: 1-588; Coverage: 100%)
Gene names: TFC8, YPL007C
Pfam: Putative zinc-finger of transcription factor IIIC complex
Transcription factor tau 60 kDa subunit in PDB entry 8ffz, assembly 1, front view.
Transcription factor tau 60 kDa subunit in PDB entry 8ffz, assembly 1, side view.
Transcription factor tau 60 kDa subunit in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar proteins

Transcription factor tau 55 kDa subunit
Chain: G
Length: 435 amino acids
Theoretical weight: 49.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12415 (Residues: 1-435; Coverage: 100%)
Gene names: O3234, TFC7, YOR110W, YOR3234w
Pfam:
Transcription factor tau 55 kDa subunit in PDB entry 8ffz, assembly 1, front view.
Transcription factor tau 55 kDa subunit in PDB entry 8ffz, assembly 1, side view.
Transcription factor tau 55 kDa subunit in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar proteins

TATA-box-binding protein; Transcription factor IIIB 70 kDa subunit
Chain: H
Length: 736 amino acids
Theoretical weight: 82.1 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P29056 (Residues: 1-382, 439-596; Coverage: 91%)
  • Canonical: P13393 (Residues: 61-240; Coverage: 75%)
    • nullnull
Gene names: BRF1, BTF1, PCF4, SPT15, TBP1, TDS4, YER148W, YGR246C
Pfam:
TATA-box-binding protein in PDB entry 8ffz, assembly 1, front view.
TATA-box-binding protein in PDB entry 8ffz, assembly 1, side view.
TATA-box-binding protein in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar proteins

DNA chain I
Name: DNA (151-MER)
Representative chains: I
Length: 171 nucleotides
Theoretical weight: 52.64 KDa
Rfam: 5S ribosomal RNA
DNA (151-MER) in PDB entry 8ffz, assembly 1, front view.
DNA (151-MER) in PDB entry 8ffz, assembly 1, side view.
DNA (151-MER) in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar DNA

DNA chain J
Name: DNA (151-MER)
Representative chains: J
Length: 171 nucleotides
Theoretical weight: 52.93 KDa
DNA (151-MER) in PDB entry 8ffz, assembly 1, front view.
DNA (151-MER) in PDB entry 8ffz, assembly 1, side view.
DNA (151-MER) in PDB entry 8ffz, assembly 1, top view.
Molecule details ›

Search similar DNA

Quick links