Structure analysis

Yeast 40S-eIF4B - partially open conformation of the 40S head

Electron Microscopy
4.6Å resolution
Assembly composition:
hetero 35-mer (preferred)
Entry contents: 34 distinct polypeptide molecules
1 distinct RNA molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 35-mer
Accessible surface area: 421428.27 Å2
Buried surface area: 181499.68 Å2
Dissociation area: 1,424.07 Å2
Dissociation energy (ΔGdiss): -0.8 kcal/mol
Dissociation entropy (TΔSdiss): 11.47 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-271143

Macromolecules

Chain: A
Length: 254 amino acids
Theoretical weight: 28.26 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CN12 (Residues: 1-254; Coverage: 100%)
Gene names: KLLA0E16171g, RPS0
Pfam: Ribosomal protein S2

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Chain: B
Length: 255 amino acids
Theoretical weight: 28.97 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CWD0 (Residues: 1-255; Coverage: 100%)
Gene names: KLLA0B05060g, RPS1
Pfam: Ribosomal S3Ae family

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Chain: C
Length: 259 amino acids
Theoretical weight: 27.65 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CKL3 (Residues: 1-259; Coverage: 100%)
Gene name: KLLA0_F09812g
Pfam:

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Chain: D
Length: 237 amino acids
Theoretical weight: 26.3 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CRK7 (Residues: 1-237; Coverage: 100%)
Gene name: KLLA0_D08305g
Pfam:

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Chain: E
Length: 261 amino acids
Theoretical weight: 29.62 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CWJ2 (Residues: 1-261; Coverage: 100%)
Gene name: KLLA0_B03652g
Pfam:

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Chain: F
Length: 227 amino acids
Theoretical weight: 25.39 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CRA3 (Residues: 1-227; Coverage: 100%)
Gene name: KLLA0_D10659g
Pfam: Ribosomal protein S7p/S5e

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Chain: G
Length: 236 amino acids
Theoretical weight: 26.97 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CM04 (Residues: 1-236; Coverage: 100%)
Gene names: KLLA0E24090g, RPS6
Pfam: Ribosomal protein S6e

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Chain: H
Length: 190 amino acids
Theoretical weight: 21.74 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CTD6 (Residues: 1-190; Coverage: 100%)
Gene name: KLLA0_C13519g
Pfam: Ribosomal protein S7e

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Chain: I
Length: 201 amino acids
Theoretical weight: 22.64 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CMG3 (Residues: 1-201; Coverage: 100%)
Gene name: KLLA0_E20461g
Pfam: Ribosomal protein S8e

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Chain: J
Length: 188 amino acids
Theoretical weight: 21.59 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CM18 (Residues: 1-188; Coverage: 100%)
Gene name: KLLA0_E23673g
Pfam:

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Chain: K
Length: 106 amino acids
Theoretical weight: 12.58 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CVZ5 (Residues: 1-106; Coverage: 100%)
Gene name: KLLA0_B08173g
Pfam: Plectin/S10 domain

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Chain: L
Length: 156 amino acids
Theoretical weight: 17.84 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CX80 (Residues: 1-156; Coverage: 100%)
Gene name: KLLA0_A10483g
Pfam:

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Chain: M
Length: 134 amino acids
Theoretical weight: 14.47 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CLU4 (Residues: 1-134; Coverage: 100%)
Gene name: KLLA0_F00352g
Pfam: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family

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Chain: N
Length: 151 amino acids
Theoretical weight: 16.99 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CJK0 (Residues: 1-151; Coverage: 100%)
Gene name: KLLA0_F18040g
Pfam:

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Chain: O
Length: 137 amino acids
Theoretical weight: 14.53 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: P27069 (Residues: 1-137; Coverage: 100%)
Gene names: CRY1, KLLA0B07623g, RPS14
Pfam: Ribosomal protein S11

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Chain: P
Length: 142 amino acids
Theoretical weight: 15.99 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CKV4 (Residues: 1-142; Coverage: 100%)
Gene name: KLLA0_F07843g
Pfam: Ribosomal protein S19

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Chain: Q
Length: 143 amino acids
Theoretical weight: 15.87 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q875N2 (Residues: 1-143; Coverage: 100%)
Gene names: KLLA0E22077g, RPS16
Pfam: Ribosomal protein S9/S16

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Chain: R
Length: 136 amino acids
Theoretical weight: 15.72 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CWU3 (Residues: 1-136; Coverage: 100%)
Gene name: KLLA0_B01474g
Pfam: Ribosomal S17

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Chain: S
Length: 146 amino acids
Theoretical weight: 17.08 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CWT9 (Residues: 1-146; Coverage: 100%)
Gene name: KLLA0_B01562g
Pfam: Ribosomal protein S13/S18

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Chain: T
Length: 144 amino acids
Theoretical weight: 15.88 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CXM0 (Residues: 1-144; Coverage: 100%)
Gene name: KLLA0_A07194g
Pfam: Ribosomal protein S19e

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Chain: U
Length: 117 amino acids
Theoretical weight: 13.34 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CIM1 (Residues: 1-117; Coverage: 100%)
Gene name: KLLA0_F25542g
Pfam: Ribosomal protein S10p/S20e

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Chain: V
Length: 87 amino acids
Theoretical weight: 9.8 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CXT6 (Residues: 1-87; Coverage: 100%)
Gene names: KLLA0A05700g, RPS21
Pfam: Ribosomal protein S21e

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Chain: W
Length: 130 amino acids
Theoretical weight: 14.65 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CW21 (Residues: 1-130; Coverage: 100%)
Gene names: KLLA0B07601g, RPS22
Pfam: Ribosomal protein S8

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Chain: X
Length: 145 amino acids
Theoretical weight: 16.05 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: F2Z602 (Residues: 1-145; Coverage: 100%)
Gene name: KLLA0_B11231g
Pfam: Ribosomal protein S12/S23

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Chain: Y
Length: 135 amino acids
Theoretical weight: 15.19 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CU44 (Residues: 1-135; Coverage: 100%)
Gene name: KLLA0_C07755g
Pfam: Ribosomal protein S24e

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Chain: Z
Length: 108 amino acids
Theoretical weight: 12 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CW78 (Residues: 1-108; Coverage: 100%)
Gene name: KLLA0_B06182g
Pfam: S25 ribosomal protein

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Chain: a
Length: 119 amino acids
Theoretical weight: 13.54 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CS01 (Residues: 1-119; Coverage: 100%)
Gene name: KLLA0_D05115g
Pfam: Ribosomal protein S26e

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Chain: b
Length: 82 amino acids
Theoretical weight: 8.88 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CNL2 (Residues: 1-82; Coverage: 100%)
Gene name: KLLA0_E11661g
Pfam: Ribosomal protein S27

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Chain: c
Length: 67 amino acids
Theoretical weight: 7.55 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: P33285 (Residues: 1-67; Coverage: 100%)
Gene names: KLLA0F20383g, RPS28
Pfam: Ribosomal protein S28e

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Chain: d
Length: 56 amino acids
Theoretical weight: 6.66 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CPG3 (Residues: 1-56; Coverage: 100%)
Gene names: KLLA0E05071g, RPS29
Pfam: Ribosomal protein S14p/S29e

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Chain: e
Length: 63 amino acids
Theoretical weight: 7.14 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CUH5 (Residues: 1-63; Coverage: 100%)
Gene name: KLLA0_C04809g
Pfam: Ribosomal protein S30

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Chain: f
Length: 150 amino acids
Theoretical weight: 17.11 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: P69061 (Residues: 1-150; Coverage: 100%)
Gene names: KLLA0D18304g, ubi3
Pfam:

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Chain: g
Length: 326 amino acids
Theoretical weight: 35.83 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
UniProt:
  • Canonical: Q6CNI7 (Residues: 1-326; Coverage: 100%)
Gene name: KLLA0_E12277g
Pfam: WD domain, G-beta repeat

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Chain: h
Length: 25 amino acids
Theoretical weight: 3.35 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CX86 (Residues: 1-25; Coverage: 100%)
Gene names: D1290, RPL41A, RPL47A, YDL184C, YL41A
Pfam: Ribosomal protein L41

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Name: RNA (1780-MER)
Representative chains: 2
Length: 1799 nucleotides
Theoretical weight: 579.55 KDa
Rfam: Eukaryotic small subunit ribosomal RNA

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