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Chaetomium thermophilum Get1/Get2 heterotetramer in complex with a Get3 dimer (amphipol)

Electron Microscopy
5Å resolution
PDB entry 8odu coloured by chain, front view.
PDB entry 8odu coloured by chain, side view.
PDB entry 8odu coloured by chain, top view.
Source organism: Thermochaetoides thermophila DSM 1495
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Protein GET1 and Anion-transporting ATPase-like domain-containing protein
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Multimeric state: hetero tetramer
Accessible surface area: 67628.26 Å2
Buried surface area: 6610.28 Å2
Dissociation area: 2,363.17 Å2
Dissociation energy (ΔGdiss): 0.9 kcal/mol
Dissociation entropy (TΔSdiss): 28.66 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-236038

Macromolecules

Anion-transporting ATPase-like domain-containing protein
Chains: A, B
Length: 334 amino acids
Theoretical weight: 37.13 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SFE0 (Residues: 14-339; Coverage: 96%)
Gene name: CTHT_0061710
Pfam: Anion-transporting ATPase
Anion-transporting ATPase-like domain-containing protein in PDB entry 8odu, assembly 1, front view.
Anion-transporting ATPase-like domain-containing protein in PDB entry 8odu, assembly 1, side view.
Anion-transporting ATPase-like domain-containing protein in PDB entry 8odu, assembly 1, top view.
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Protein GET1
Chains: C, D
Length: 409 amino acids
Theoretical weight: 45.57 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: G0RZE4 (Residues: 185-357; Coverage: 49%)
  • Canonical: G0S1H2 (Residues: 1-209; Coverage: 100%)
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Gene names: CTHT_0002670, CTHT_0013590, GET1
Pfam:
Protein GET1 in PDB entry 8odu, assembly 1, front view.
Protein GET1 in PDB entry 8odu, assembly 1, side view.
Protein GET1 in PDB entry 8odu, assembly 1, top view.
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