Structure analysis

Bacillus subtilis MutS2-collided disome complex (collided 70S)

Electron Microscopy
3.57Å resolution
Source organism: Bacillus subtilis
Assembly composition:
hetero 53-mer (preferred)
Entry contents: 47 distinct polypeptide molecules
6 distinct RNA molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 53-mer
Accessible surface area: 793190.4 Å2
Buried surface area: 243107.94 Å2
Dissociation area: 1,894.17 Å2
Dissociation energy (ΔGdiss): 7.66 kcal/mol
Dissociation entropy (TΔSdiss): 14.06 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-281408

Macromolecules

Chain: 0
Length: 59 amino acids
Theoretical weight: 6.75 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: O34687 (Residues: 1-59; Coverage: 100%)
Gene names: BSU15080, rpmF
Pfam: Ribosomal L32p protein family

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Chain: 1
Length: 48 amino acids
Theoretical weight: 5.79 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P56849 (Residues: 1-48; Coverage: 98%)
Gene names: BSU24900, rpmG1, rpmGA
Pfam: Ribosomal protein L33

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Chain: 2
Length: 44 amino acids
Theoretical weight: 5.27 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P05647 (Residues: 1-44; Coverage: 100%)
Gene names: BSU41060, rpmH
Pfam: Ribosomal protein L34

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Chain: 3
Length: 66 amino acids
Theoretical weight: 7.6 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P55874 (Residues: 1-66; Coverage: 100%)
Gene names: BSU28860, rpmI
Pfam: Ribosomal protein L35

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Chain: 4
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P20278 (Residues: 1-37; Coverage: 100%)
Gene names: BSU01400, rpmJ
Pfam: Ribosomal protein L36

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Chain: 6
Length: 64 amino acids
Theoretical weight: 7.22 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: Q03223 (Residues: 1-64; Coverage: 97%)
Gene names: BSU37070, rpmE
Pfam: Ribosomal protein L31

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Chain: B
Length: 246 amino acids
Theoretical weight: 28.01 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21464 (Residues: 1-246; Coverage: 100%)
Gene names: BSU16490, rpsB
Pfam: Ribosomal protein S2

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Chain: C
Length: 218 amino acids
Theoretical weight: 24.35 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21465 (Residues: 1-218; Coverage: 100%)
Gene names: BSU01220, rpsC
Pfam:

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Chain: D
Length: 200 amino acids
Theoretical weight: 22.87 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21466 (Residues: 1-200; Coverage: 100%)
Gene names: BSU29660, rpsD
Pfam:

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Chain: E
Length: 166 amino acids
Theoretical weight: 17.65 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21467 (Residues: 1-166; Coverage: 100%)
Gene names: BSU01330, rpsE, spcA
Pfam:

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Chain: F
Length: 95 amino acids
Theoretical weight: 11.14 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21468 (Residues: 1-95; Coverage: 100%)
Gene names: BSU40910, rpsF
Pfam: Ribosomal protein S6

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Chain: G
Length: 156 amino acids
Theoretical weight: 17.92 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21469 (Residues: 1-156; Coverage: 100%)
Gene names: BSU01110, rpsG
Pfam: Ribosomal protein S7p/S5e

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Chain: H
Length: 132 amino acids
Theoretical weight: 14.9 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P12879 (Residues: 1-132; Coverage: 100%)
Gene names: BSU01300, rpsH
Pfam: Ribosomal protein S8

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Chain: I
Length: 130 amino acids
Theoretical weight: 14.34 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21470 (Residues: 1-130; Coverage: 100%)
Gene names: BSU01500, rpsI
Pfam: Ribosomal protein S9/S16

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Chain: J
Length: 102 amino acids
Theoretical weight: 11.69 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21471 (Residues: 1-102; Coverage: 100%)
Gene names: BSU01150, rpsJ, tetA
Pfam: Ribosomal protein S10p/S20e

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Chain: K
Length: 131 amino acids
Theoretical weight: 13.99 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P04969 (Residues: 1-131; Coverage: 100%)
Gene names: BSU01420, rpsK
Pfam: Ribosomal protein S11

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Chain: L
Length: 138 amino acids
Theoretical weight: 15.25 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21472 (Residues: 1-138; Coverage: 100%)
Gene names: BSU01100, fun, rpsL, strA
Pfam: Ribosomal protein S12/S23

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Chain: M
Length: 121 amino acids
Theoretical weight: 13.82 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P20282 (Residues: 1-121; Coverage: 100%)
Gene names: BSU01410, rpsM
Pfam: Ribosomal protein S13/S18

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Chain: N
Length: 61 amino acids
Theoretical weight: 7.26 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P12878 (Residues: 1-61; Coverage: 100%)
Gene names: BSU01290, rpsN, rpsN1, rpsNA, rpsZ
Pfam: Ribosomal protein S14p/S29e

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Chain: O
Length: 89 amino acids
Theoretical weight: 10.6 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21473 (Residues: 1-89; Coverage: 100%)
Gene names: BSU16680, rpsO
Pfam: Ribosomal protein S15

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Chain: P
Length: 90 amino acids
Theoretical weight: 10.15 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21474 (Residues: 1-90; Coverage: 100%)
Gene names: BSU15990, rpsP
Pfam: Ribosomal protein S16

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Chain: Q
Length: 87 amino acids
Theoretical weight: 10.22 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P12874 (Residues: 1-87; Coverage: 100%)
Gene names: BSU01250, rpsQ
Pfam: Ribosomal protein S17

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Chain: R
Length: 79 amino acids
Theoretical weight: 8.99 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21475 (Residues: 1-79; Coverage: 100%)
Gene names: BSU40890, rpsR
Pfam: Ribosomal protein S18

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Chain: S
Length: 92 amino acids
Theoretical weight: 10.61 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21476 (Residues: 1-92; Coverage: 100%)
Gene names: BSU01200, rpsS
Pfam: Ribosomal protein S19

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Chain: T
Length: 88 amino acids
Theoretical weight: 9.62 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P21477 (Residues: 1-88; Coverage: 100%)
Gene names: BSU25550, rpsT, yqeO
Pfam: Ribosomal protein S20

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Chain: Z
Length: 275 amino acids
Theoretical weight: 30.07 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P42919 (Residues: 2-276; Coverage: 99%)
Gene names: BSU01190, rplB
Pfam:

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Chain: a
Length: 207 amino acids
Theoretical weight: 22.46 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P42920 (Residues: 2-208; Coverage: 99%)
Gene names: BSU01160, rplC
Pfam: Ribosomal protein L3

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Chain: b
Length: 205 amino acids
Theoretical weight: 22.22 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P42921 (Residues: 2-206; Coverage: 99%)
Gene names: BSU01170, rplD
Pfam: Ribosomal protein L4/L1 family

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Chain: c
Length: 178 amino acids
Theoretical weight: 20.05 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P12877 (Residues: 2-179; Coverage: 99%)
Gene names: BSU01280, rplE
Pfam:

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Chain: d
Length: 175 amino acids
Theoretical weight: 19.12 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P46898 (Residues: 2-176; Coverage: 98%)
Gene names: BSU01310, rplF
Pfam: Ribosomal protein L6

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Chain: e
Length: 142 amino acids
Theoretical weight: 16.02 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P70974 (Residues: 4-145; Coverage: 98%)
Gene names: BSU01490, rplM
Pfam: Ribosomal protein L13

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Chain: f
Length: 122 amino acids
Theoretical weight: 13.18 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P12875 (Residues: 1-122; Coverage: 100%)
Gene names: BSU01260, rplN
Pfam: Ribosomal protein L14p/L23e

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Chain: i
Length: 146 amino acids
Theoretical weight: 15.41 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P19946 (Residues: 1-146; Coverage: 100%)
Gene names: BSU01350, rplO
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A

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Chain: j
Length: 138 amino acids
Theoretical weight: 15.62 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P14577 (Residues: 1-138; Coverage: 96%)
Gene names: BSU01230, rplP
Pfam: Ribosomal protein L16p/L10e

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Chain: k
Length: 119 amino acids
Theoretical weight: 13.64 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P20277 (Residues: 2-120; Coverage: 99%)
Gene names: BSU01440, rplQ
Pfam: Ribosomal protein L17

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Chain: l
Length: 120 amino acids
Theoretical weight: 12.99 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P46899 (Residues: 1-120; Coverage: 100%)
Gene names: BSU01320, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast

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Chain: m
Length: 115 amino acids
Theoretical weight: 13.42 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: O31742 (Residues: 1-115; Coverage: 100%)
Gene names: BSU16040, rplS
Pfam: Ribosomal protein L19

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Chain: n
Length: 117 amino acids
Theoretical weight: 13.41 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P55873 (Residues: 2-118; Coverage: 98%)
Gene names: BSU28850, rplT
Pfam: Ribosomal protein L20

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Chain: o
Length: 101 amino acids
Theoretical weight: 11.16 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P26908 (Residues: 2-102; Coverage: 99%)
Gene names: BSU27960, rplU
Pfam: Ribosomal prokaryotic L21 protein

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Chain: r
Length: 109 amino acids
Theoretical weight: 12.04 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P42060 (Residues: 2-110; Coverage: 97%)
Gene names: BSU01210, rplV
Pfam: Ribosomal protein L22p/L17e

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Chain: s
Length: 93 amino acids
Theoretical weight: 10.78 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P42924 (Residues: 1-93; Coverage: 98%)
Gene names: BSU01180, rplW
Pfam: Ribosomal protein L23

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Chain: t
Length: 101 amino acids
Theoretical weight: 10.92 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P0CI78 (Residues: 1-101; Coverage: 98%)
Gene names: BSU01270, rplX
Pfam:

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Chain: u
Length: 82 amino acids
Theoretical weight: 8.96 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P05657 (Residues: 11-92; Coverage: 87%)
Gene names: BSU27940, rpmA
Pfam: Ribosomal L27 protein

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Chain: v
Length: 58 amino acids
Theoretical weight: 6.37 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P37807 (Residues: 3-60; Coverage: 94%)
Gene names: BSU15820, rpmB, yloT
Pfam: Ribosomal L28 family

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Chain: w
Length: 65 amino acids
Theoretical weight: 7.6 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P12873 (Residues: 1-65; Coverage: 99%)
Gene names: BSU01240, rpmC
Pfam: Ribosomal L29 protein

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Chain: x
Length: 58 amino acids
Theoretical weight: 6.52 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P19947 (Residues: 2-59; Coverage: 98%)
Gene names: BSU01340, rpmD
Pfam: Ribosomal protein L30p/L7e

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Chain: z
Length: 785 amino acids
Theoretical weight: 87.53 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P94545 (Residues: 1-785; Coverage: 100%)
Gene names: BSU28580, mutS2, mutSB, rqcU, yshD
Pfam:

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Name: tRNA (73-MER)
Representative chains: 7
Length: 73 nucleotides
Theoretical weight: 23.55 KDa
Rfam: tRNA

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Name: 16S rRNA (1533-MER)
Representative chains: A
Length: 1533 nucleotides
Theoretical weight: 496.85 KDa
Rfam: Bacterial small subunit ribosomal RNA

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Name: tRNA (77-MER)
Representative chains: U
Length: 77 nucleotides
Theoretical weight: 24.82 KDa
Rfam: tRNA

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Name: mRNA (33-MER)
Representative chains: V
Length: 33 nucleotides
Theoretical weight: 10.59 KDa

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Name: 5S rRNA (112-MER)
Representative chains: Y
Length: 112 nucleotides
Theoretical weight: 36.16 KDa
Rfam: 5S ribosomal RNA

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Name: 23S RNA (2887-MER)
Representative chains: X
Length: 2928 nucleotides
Theoretical weight: 949.65 KDa
Rfam: Bacterial large subunit ribosomal RNA

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