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Cryo- EM structure of Mycobacterium smegmatis 70S ribosome, bS1 and RafH.

Electron Microscopy
3.5Å resolution
PDB entry 8wi7 coloured by chain, front view.
PDB entry 8wi7 coloured by chain, side view.
PDB entry 8wi7 coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Assembly composition:
hetero 51-mer (preferred)
Entry contents: 48 distinct polypeptide molecules
3 distinct RNA molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero 51-mer
Accessible surface area: 798294.69 Å2
Buried surface area: 239001.51 Å2
Dissociation area: 1,806.46 Å2
Dissociation energy (ΔGdiss): -10.29 kcal/mol
Dissociation entropy (TΔSdiss): 23.8 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-279084

Macromolecules

Large ribosomal subunit protein uL2
Chain: E
Length: 278 amino acids
Theoretical weight: 30.43 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD4 (Residues: 1-278; Coverage: 100%)
Gene names: MSMEG_1439, MSMEI_1403, rplB
Pfam:
Large ribosomal subunit protein uL2 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL2 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL2 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL3
Chain: F
Length: 217 amino acids
Theoretical weight: 23.01 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD1 (Residues: 1-217; Coverage: 100%)
Gene names: MSMEG_1436, MSMEI_1400, rplC
Pfam: Ribosomal protein L3
Large ribosomal subunit protein uL3 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL3 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL3 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL4
Chain: G
Length: 215 amino acids
Theoretical weight: 22.92 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD2 (Residues: 1-215; Coverage: 100%)
Gene names: MSMEG_1437, MSMEI_1401, rplD
Pfam: Ribosomal protein L4/L1 family
Large ribosomal subunit protein uL4 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL4 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL4 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL5
Chain: H
Length: 187 amino acids
Theoretical weight: 21.15 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSG1 (Residues: 1-187; Coverage: 100%)
Gene names: MSMEG_1467, MSMEI_1431, rplE
Pfam:
Large ribosomal subunit protein uL5 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL5 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL5 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL6
Chain: I
Length: 179 amino acids
Theoretical weight: 19.48 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSG4 (Residues: 1-179; Coverage: 100%)
Gene names: MSMEG_1470, MSMEI_1434, rplF
Pfam: Ribosomal protein L6
Large ribosomal subunit protein uL6 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL6 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL6 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL9
Chain: J
Length: 151 amino acids
Theoretical weight: 15.95 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R7F6 (Residues: 1-151; Coverage: 100%)
Gene names: MSMEG_6894, MSMEI_6710, rplI
Pfam:
Large ribosomal subunit protein bL9 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL9 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL9 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL13
Chain: M
Length: 147 amino acids
Theoretical weight: 16.15 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSP8 (Residues: 1-147; Coverage: 100%)
Gene names: MSMEG_1556, MSMEI_1519, rplM
Pfam: Ribosomal protein L13
Large ribosomal subunit protein uL13 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL13 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL13 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL14
Chain: N
Length: 122 amino acids
Theoretical weight: 13.4 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSF9 (Residues: 1-122; Coverage: 100%)
Gene names: MSMEG_1465, MSMEI_1429, rplN
Pfam: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL14 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL14 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL14 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL15
Chain: O
Length: 147 amino acids
Theoretical weight: 15.6 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSG8 (Residues: 1-147; Coverage: 100%)
Gene names: MSMEG_1474, rplO
Pfam: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL15 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL15 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL15 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL17
Chain: Q
Length: 199 amino acids
Theoretical weight: 21.89 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSL9 (Residues: 1-199; Coverage: 100%)
Gene names: MSMEG_1525, MSMEI_1489, rplQ
Pfam: Ribosomal protein L17
Large ribosomal subunit protein bL17 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL17 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL17 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL18
Chain: R
Length: 127 amino acids
Theoretical weight: 13.71 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSG5 (Residues: 1-127; Coverage: 100%)
Gene names: MSMEG_1471, rplR
Pfam: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Large ribosomal subunit protein uL18 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL18 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL18 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL19
Chain: S
Length: 113 amino acids
Theoretical weight: 12.89 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QV42 (Residues: 1-113; Coverage: 100%)
Gene names: MSMEG_2440, MSMEI_2379, rplS
Pfam: Ribosomal protein L19
Large ribosomal subunit protein bL19 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL19 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL19 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL20
Chain: T
Length: 129 amino acids
Theoretical weight: 14.49 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QYU6 (Residues: 1-129; Coverage: 100%)
Gene names: MSMEG_3791, MSMEI_3703, rplT
Pfam: Ribosomal protein L20
Large ribosomal subunit protein bL20 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL20 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL20 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL21
Chain: U
Length: 103 amino acids
Theoretical weight: 11.06 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R151 (Residues: 1-103; Coverage: 100%)
Gene names: MSMEG_4625, MSMEI_4508, rplU
Pfam: Ribosomal prokaryotic L21 protein
Large ribosomal subunit protein bL21 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL21 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL21 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL22
Chain: V
Length: 153 amino acids
Theoretical weight: 16.35 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD6 (Residues: 1-153; Coverage: 100%)
Gene names: MSMEG_1441, MSMEI_1405, rplV
Pfam: Ribosomal protein L22p/L17e
Large ribosomal subunit protein uL22 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL22 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL22 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL23
Chain: W
Length: 100 amino acids
Theoretical weight: 11.05 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD3 (Residues: 1-100; Coverage: 100%)
Gene names: MSMEG_1438, MSMEI_1402, rplW
Pfam: Ribosomal protein L23
Large ribosomal subunit protein uL23 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL23 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL23 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL24
Chain: X
Length: 105 amino acids
Theoretical weight: 11.23 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSG0 (Residues: 1-105; Coverage: 100%)
Gene names: MSMEG_1466, MSMEI_1430, rplX
Pfam:
Large ribosomal subunit protein uL24 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL24 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL24 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL27
Chain: Z
Length: 88 amino acids
Theoretical weight: 9.25 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R150 (Residues: 1-88; Coverage: 100%)
Gene names: MSMEG_4624, MSMEI_4507, rpmA
Pfam: Ribosomal L27 protein
Large ribosomal subunit protein bL27 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL27 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL27 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL28
Chain: 1
Length: 64 amino acids
Theoretical weight: 6.89 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QV03 (Residues: 1-64; Coverage: 100%)
Gene names: MSMEG_2400, rpmB
Pfam: Ribosomal L28 family
Large ribosomal subunit protein bL28 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL28 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL28 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL29
Chain: 2
Length: 77 amino acids
Theoretical weight: 8.79 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD9 (Residues: 1-77; Coverage: 100%)
Gene names: MSMEG_1444, MSMEI_1408, rpmC
Pfam: Ribosomal L29 protein
Large ribosomal subunit protein uL29 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL29 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL29 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein uL30
Chain: 3
Length: 61 amino acids
Theoretical weight: 7.02 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSG7 (Residues: 1-61; Coverage: 100%)
Gene names: MSMEG_1473, MSMEI_1437, rpmD
Pfam: Ribosomal protein L30p/L7e
Large ribosomal subunit protein uL30 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein uL30 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein uL30 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL32
Chain: 5
Length: 57 amino acids
Theoretical weight: 6.47 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R3I9 (Residues: 1-57; Coverage: 100%)
Gene names: MSMEG_5489, MSMEI_5337, rpmF
Pfam: Ribosomal L32p protein family
Large ribosomal subunit protein bL32 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL32 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL32 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL33A
Chain: 6
Length: 55 amino acids
Theoretical weight: 6.47 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QS39 (Residues: 1-55; Coverage: 100%)
Gene names: MSMEG_1339, MSMEI_1301, rpmG1, rpmG2
Pfam: Ribosomal protein L33
Large ribosomal subunit protein bL33A in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL33A in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL33A in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL34
Chain: 7
Length: 47 amino acids
Theoretical weight: 5.52 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R7K0 (Residues: 1-47; Coverage: 100%)
Gene names: MSMEG_6946, MSMEI_6752, rpmH
Pfam: Ribosomal protein L34
Large ribosomal subunit protein bL34 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL34 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL34 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL35
Chain: 8
Length: 64 amino acids
Theoretical weight: 7.3 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QYU7 (Residues: 1-64; Coverage: 100%)
Gene names: MSMEG_3792, MSMEI_3704, rpmI
Pfam: Ribosomal protein L35
Large ribosomal subunit protein bL35 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL35 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL35 in PDB entry 8wi7, assembly 1, top view.
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Large ribosomal subunit protein bL31
Chain: 4
Length: 75 amino acids
Theoretical weight: 8.31 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R215 (Residues: 1-75; Coverage: 100%)
Gene names: MSMEG_4951, MSMEI_4823, rpmE
Pfam: Ribosomal protein L31
Large ribosomal subunit protein bL31 in PDB entry 8wi7, assembly 1, front view.
Large ribosomal subunit protein bL31 in PDB entry 8wi7, assembly 1, side view.
Large ribosomal subunit protein bL31 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein bS1
Chain: b
Length: 479 amino acids
Theoretical weight: 53.38 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QYY6 (Residues: 1-479; Coverage: 100%)
Gene names: LJ00_19040, MSMEG_3833, MSMEI_3743, rpsA
Pfam: S1 RNA binding domain
Small ribosomal subunit protein bS1 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein bS1 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein bS1 in PDB entry 8wi7, assembly 1, top view.
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Ribosomal protein mS38 C-terminal domain-containing protein
Chain: v
Length: 33 amino acids
Theoretical weight: 4.16 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QR10 (Residues: 1-33; Coverage: 100%)
Gene name: MSMEG_0945
Pfam: Mitochondrial mRNA-processing protein COX24, C-terminal
Ribosomal protein mS38 C-terminal domain-containing protein in PDB entry 8wi7, assembly 1, front view.
Ribosomal protein mS38 C-terminal domain-containing protein in PDB entry 8wi7, assembly 1, side view.
Ribosomal protein mS38 C-terminal domain-containing protein in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS3
Chain: d
Length: 275 amino acids
Theoretical weight: 30.19 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD7 (Residues: 1-275; Coverage: 100%)
Gene names: MSMEG_1442, MSMEI_1406, rpsC
Pfam:
Small ribosomal subunit protein uS3 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS3 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS3 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS4
Chain: e
Length: 201 amino acids
Theoretical weight: 23.42 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSL7 (Residues: 1-201; Coverage: 100%)
Gene names: MSMEG_1523, MSMEI_1487, rpsD
Pfam:
Small ribosomal subunit protein uS4 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS4 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS4 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS5
Chain: f
Length: 214 amino acids
Theoretical weight: 21.95 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSG6 (Residues: 1-214; Coverage: 100%)
Gene names: MSMEG_1472, MSMEI_1436, rpsE
Pfam:
Small ribosomal subunit protein uS5 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS5 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS5 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein bS6
Chain: g
Length: 96 amino acids
Theoretical weight: 10.99 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R7F9 (Residues: 1-90; Coverage: 100%)
Gene names: MSMEG_6897, MSMEI_6713, rpsF
Pfam: Ribosomal protein S6
Small ribosomal subunit protein bS6 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein bS6 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein bS6 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS7
Chain: h
Length: 156 amino acids
Theoretical weight: 17.66 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QS97 (Residues: 1-156; Coverage: 100%)
Gene names: MSMEG_1399, MSMEI_1361, rpsG
Pfam: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS7 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS7 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS7 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS8
Chain: i
Length: 132 amino acids
Theoretical weight: 14.49 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSG3 (Residues: 1-132; Coverage: 100%)
Gene names: MSMEG_1469, MSMEI_1433, rpsH
Pfam: Ribosomal protein S8
Small ribosomal subunit protein uS8 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS8 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS8 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS9
Chain: j
Length: 150 amino acids
Theoretical weight: 16.79 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSP9 (Residues: 1-150; Coverage: 100%)
Gene names: MSMEG_1557, MSMEI_1520, rpsI
Pfam: Ribosomal protein S9/S16
Small ribosomal subunit protein uS9 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS9 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS9 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS10
Chain: k
Length: 101 amino acids
Theoretical weight: 11.45 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD0 (Residues: 1-101; Coverage: 100%)
Gene names: MSMEG_1435, MSMEI_1399, rpsJ
Pfam: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS10 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS10 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS10 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS11
Chain: l
Length: 138 amino acids
Theoretical weight: 14.67 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSL6 (Residues: 1-138; Coverage: 100%)
Gene names: MSMEG_1522, MSMEI_1486, rpsK
Pfam: Ribosomal protein S11
Small ribosomal subunit protein uS11 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS11 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS11 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS12
Chain: m
Length: 124 amino acids
Theoretical weight: 13.9 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QS96 (Residues: 1-124; Coverage: 100%)
Gene names: MSMEG_1398, MSMEI_1360, rpsL
Pfam: Ribosomal protein S12/S23
Small ribosomal subunit protein uS12 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS12 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS12 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS13
Chain: n
Length: 124 amino acids
Theoretical weight: 14.25 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSL5 (Residues: 1-124; Coverage: 100%)
Gene names: MSMEG_1521, MSMEI_1485, rpsM
Pfam: Ribosomal protein S13/S18
Small ribosomal subunit protein uS13 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS13 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS13 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS14A
Chain: o
Length: 101 amino acids
Theoretical weight: 11.79 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R550 (Residues: 1-101; Coverage: 100%)
Gene names: MSMEG_6066, MSMEI_5906, rpsN
Pfam: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS14A in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS14A in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS14A in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS15
Chain: p
Length: 89 amino acids
Theoretical weight: 10.37 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QVQ3 (Residues: 1-89; Coverage: 100%)
Gene names: MSMEG_2654, MSMEI_2591, rpsO
Pfam: Ribosomal protein S15
Small ribosomal subunit protein uS15 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS15 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS15 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein bS16
Chain: q
Length: 156 amino acids
Theoretical weight: 16.8 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QV37 (Residues: 1-156; Coverage: 100%)
Gene names: MSMEG_2435, MSMEI_2374, rpsP
Pfam: Ribosomal protein S16
Small ribosomal subunit protein bS16 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein bS16 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein bS16 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS17
Chain: r
Length: 98 amino acids
Theoretical weight: 11.13 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSE0 (Residues: 1-98; Coverage: 100%)
Gene names: MSMEG_1445, MSMEI_1409, rpsQ
Pfam: Ribosomal protein S17
Small ribosomal subunit protein uS17 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS17 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS17 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein bS18B
Chain: s
Length: 84 amino acids
Theoretical weight: 9.52 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R7F7 (Residues: 1-84; Coverage: 100%)
Gene names: MSMEG_6895, MSMEI_6711, rpsR1, rpsR2
Pfam: Ribosomal protein S18
Small ribosomal subunit protein bS18B in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein bS18B in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein bS18B in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS19
Chain: t
Length: 93 amino acids
Theoretical weight: 10.8 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QSD5 (Residues: 1-93; Coverage: 100%)
Gene names: MSMEG_1440, MSMEI_1404, rpsS
Pfam: Ribosomal protein S19
Small ribosomal subunit protein uS19 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS19 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS19 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein bS20
Chain: u
Length: 86 amino acids
Theoretical weight: 9.56 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0R102 (Residues: 1-86; Coverage: 100%)
Gene names: MSMEG_4571, MSMEI_4459, rpsT
Pfam: Ribosomal protein S20
Small ribosomal subunit protein bS20 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein bS20 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein bS20 in PDB entry 8wi7, assembly 1, top view.
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Small ribosomal subunit protein uS2
Chain: c
Length: 277 amino acids
Theoretical weight: 30.15 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
UniProt:
  • Canonical: A0QVB8 (Residues: 1-277; Coverage: 100%)
Gene names: MSMEG_2519, MSMEI_2460, rpsB
Pfam: Ribosomal protein S2
Small ribosomal subunit protein uS2 in PDB entry 8wi7, assembly 1, front view.
Small ribosomal subunit protein uS2 in PDB entry 8wi7, assembly 1, side view.
Small ribosomal subunit protein uS2 in PDB entry 8wi7, assembly 1, top view.
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Sigma 54 modulation/S30EA ribosomal protein C-terminal domain-containing protein
Chain: w
Length: 264 amino acids
Theoretical weight: 29.9 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0QZ86 (Residues: 1-258; Coverage: 100%)
Gene name: MSMEG_3935
Pfam:
Sigma 54 modulation/S30EA ribosomal protein C-terminal domain-containing protein in PDB entry 8wi7, assembly 1, front view.
Sigma 54 modulation/S30EA ribosomal protein C-terminal domain-containing protein in PDB entry 8wi7, assembly 1, side view.
Sigma 54 modulation/S30EA ribosomal protein C-terminal domain-containing protein in PDB entry 8wi7, assembly 1, top view.
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RNA chain A
Name: 23S ribosomal RNA
Representative chains: A
Length: 3119 nucleotides
Theoretical weight: 1011.83 KDa
Rfam: Bacterial large subunit ribosomal RNA
23S ribosomal RNA in PDB entry 8wi7, assembly 1, front view.
23S ribosomal RNA in PDB entry 8wi7, assembly 1, side view.
23S ribosomal RNA in PDB entry 8wi7, assembly 1, top view.
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RNA chain B
Name: 5S ribosomal RNA
Representative chains: B
Length: 118 nucleotides
Theoretical weight: 38.06 KDa
Rfam: 5S ribosomal RNA
5S ribosomal RNA in PDB entry 8wi7, assembly 1, front view.
5S ribosomal RNA in PDB entry 8wi7, assembly 1, side view.
5S ribosomal RNA in PDB entry 8wi7, assembly 1, top view.
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RNA chain a
Name: 16S ribosomal RNA
Representative chains: a
Length: 1528 nucleotides
Theoretical weight: 495.37 KDa
Rfam: Bacterial small subunit ribosomal RNA
16S ribosomal RNA in PDB entry 8wi7, assembly 1, front view.
16S ribosomal RNA in PDB entry 8wi7, assembly 1, side view.
16S ribosomal RNA in PDB entry 8wi7, assembly 1, top view.
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