Structure analysis

Cryo-EM structure of the ClpP degradation system in Streptomyces hawaiiensis

Electron Microscopy
2.34Å resolution
Source organism: Streptomyces hawaiiensis
Assembly composition:
hetero tetradecamer (preferred)
Entry contents: 2 distinct polypeptide molecules

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero tetradecamer
Accessible surface area: 96655.08 Å2
Buried surface area: 47525.47 Å2
Dissociation area: 6,279.76 Å2
Dissociation energy (ΔGdiss): 72.8 kcal/mol
Dissociation entropy (TΔSdiss): 16.71 kcal/mol
Symmetry number: 7
PDBe Complex ID: PDB-CPX-242121

Macromolecules

Chains: A, B, C, D, E, F, G
Length: 210 amino acids
Theoretical weight: 22.63 KDa
Source organism: Streptomyces hawaiiensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5B9BGY8 (Residues: 31-219; Coverage: 86%)
Gene names: CEB94_14110, clpP, clpP1
Pfam: Clp protease

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Chains: H, I, J, K, L, M, N
Length: 220 amino acids
Theoretical weight: 24.04 KDa
Source organism: Streptomyces hawaiiensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5B9BIX9 (Residues: 52-235; Coverage: 78%)
Gene names: CEB94_14105, clpP, clpP2
Pfam: Clp protease

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