$VAR1 = undef;

Summary for peptidase C01.005: stem bromelain

Names
MEROPS Name	stem bromelain
Other names	Ananase, bromelain, Nexobrid

Domain architecture

MEROPS Classification
Classification	Clan CA >> Subclan (none) >> Family C1 >> Subfamily A >> C01.005
Holotype	stem bromelain (Ananas comosus), Uniprot accession P14518 (peptidase unit: 2-211), MERNUM MER0000709
History	Identifier created: Handbook of Proteolytic Enzymes (1998) Academic Press, London.

Activity
Catalytic type	Cysteine
Peplist	Included in the Peplist with identifier PL00050
NC-IUBMB	Subclass 3.4 (Peptidases) >> Sub-subclass 3.4.22 (Cysteine endopeptidases) >> Peptidase 3.4.22.32
Enzymology	BRENDA database
Biotechnology	Laboratory reagent in blood group determination.
Pharmaceutical relevance	Bromelain may be effective for burn debridement (Rowan et al., 1990). It may also be useful to activate inflammatory response or as vaccine adjuvant (Engwerda et al.%, 2001; Engwerda et al.%, 2001). It has been suggested to be useful in the treatment of osteoarthritis (Brien et al., 2004). Bromelain has been shown to improve the decrease in defecation in postoperative rats, and it is suggested that bromelain may benefit patients with postoperative ileus (Wen et al., 2005).
Other databases	WIKIPEDIA	http://en.wikipedia.org/wiki/Bromelain
Cleavage site specificity	Explanations of how to interpret the following cleavage site sequence logo and specificity matrix can be found here.
Cleavage pattern	-/pl/-/--/-/-/- (based on 26 cleavages)

Specificity matrix

Amino acid	P4	P3	P2	P1	P1'	P2'	P3'	P4'
Gly	0	1	3	2	2	0	1	3
Pro	4	6	1	0	0	3	2	2
Ala	1	1	0	2	0	2	0	1
Val	0	1	3	2	2	1	1	1
Leu	2	5	4	3	1	2	3	1
Ile	1	0	0	0	1	0	0	0
Met	0	0	0	0	0	0	0	0
Phe	1	1	3	4	2	0	1	1
Tyr	2	0	1	1	1	1	0	2
Trp	0	0	0	0	0	0	0	0
Ser	2	1	2	5	6	4	3	2
Thr	1	1	1	2	2	1	1	1
Cys	1	0	0	0	0	0	1	0
Asn	0	0	1	1	1	1	2	0
Gln	0	0	0	1	1	1	1	0
Asp	0	0	0	0	1	1	1	1
Glu	2	0	0	0	1	2	2	4
Lys	0	1	0	0	1	2	0	1
Arg	3	2	4	2	0	0	1	2
His	1	1	1	0	2	2	2	1

Specificity from combinatorial peptides

Organism	comment	P4	P3	P2	P1	P1'	P2'	P3'	P4'	optimal substrate	fluorophore or acceptor-donor pair	Reference
Ananas comosus	wild type	-	G/F/V	F/L/I	R	-	-	-	-	xGFRxxxx	ACC-Dabcyl	Sun et al., 2007
Ananas comosus	wild type	G/broad	P	R	R/Q/M	-	-	-	-	GPRR	ACC	Choe et al., 2006