Structure for peptidase M16.002: insulysin

Summary Gene structure Alignment Tree Sequences Sequence features Distribution Structure Literature Substrates

PDB	Organism	Resolution	Comment
2G54	Homo sapiens	2.25 Å	complex with oxidized insulin B chain
One molecule of the homodimer is shown; only the peptidase is shown. The catalytic zinc is shown as a light grey CPK sphere. The zinc ligands are shown in ball-and-stick representation: His107 and His111 in purple, and Glu188 in dark blue. The catalytic Glu110 is shown in ball-and-stick representation in dark blue

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TERTIARY STRUCTURE DATA
Comment	Resolution	PDB	PDBe	SCOP	CATH	PDBSum	Proteopedia	Reference
Homo sapiens
complex with beta-amyloid(1-40)	2.10 Å	2G47	2G47	2G47	2G47	2G47	2G47	Shen et al., 2006
complex with amylin	2.60 Å	2G48	2G48	2G48	2G48	2G48	2G48	Shen et al., 2006
complex with glucagon	2.50 Å	2G49	2G49	2G49	2G49	2G49	2G49	Shen et al., 2006
complex with oxidized insulin B chain	2.25 Å	2G54	2G54	2G54	2G54	2G54	2G54	Shen et al., 2006
complex with oxidized insulin B chain	2.20 Å	2G56	2G56	2G56	2G56	2G56	2G56	Shen et al., 2006
Glu111Gln mutant	3.00 Å	2JBU	2JBU	2JBU	2JBU	2JBU	2JBU	Im et al., 2007
Tyr830Phe mutant	2.80 Å	2JG4	2JG4	2JG4	2JG4	2JG4	2JG4	Im et al., 2007
complex with insulin	2.60 Å	2WBY	2WBY	2WBY	2WBY	2WBY	2WBY	Manolopoulou et al., 2009
complex with iodinated insulin	2.80 Å	2WC0	2WC0	2WC0	2WC0	2WC0	2WC0	Manolopoulou et al., 2009
mature peptidase	2.59 Å	2WK3	2WK3	2WK3	2WK3	2WK3	2WK3	Guo et al., 2010
mature peptidase	2.80 Å	2YPU	2YPU	2YPU	2YPU	2YPU	2YPU	Charton et al., 2014
complex with bradykinin	1.96 Å	3CWW	3CWW	3CWW	3CWW	3CWW	3CWW	Malito et al., 2008
human ide-inhibitor complex at 2.6 resolution	2.60 Å	3E4A	3E4A	3E4A	3E4A	3E4A	3E4A	Leissring et al., 2010
mature peptidase	2.28 Å	3E4Z	3E4Z	3E4Z	3E4Z	3E4Z	3E4Z	Guo et al., 2010
mature peptidase	2.30 Å	3E50	3E50	3E50	3E50	3E50	3E50	Guo et al., 2010
mature peptidase	3.00 Å	3H44	3H44	3H44	3H44	3H44	3H44
mature peptidase	2.91 Å	3HGZ	3HGZ	3HGZ	3HGZ	3HGZ	3HGZ	Guo et al., 2010
mature peptidase	3.10 Å	3N56	3N56	3N56	3N56	3N56	3N56
mature peptidase	3.03 Å	3N57	3N57	3N57	3N57	3N57	3N57
mature peptidase	2.35 Å	3OFI	3OFI	3OFI	3OFI	3OFI	3OFI	Ralat et al., 2011
mature peptidase	3.20 Å	3QZ2	3QZ2	3QZ2	3QZ2	3QZ2	3QZ2	Charton et al., 2014
mature peptidase	3.22 Å	4DTT	4DTT	4DTT	4DTT	4DTT	4DTT	Charton et al., 2014
mature peptidase	3.00 Å	4DWK	4DWK	4DWK	4DWK	4DWK	4DWK	Charton et al., 2014
mature peptidase	2.99 Å	4GS8	4GS8	4GS8	4GS8	4GS8	4GS8	Charton et al., 2014
mature peptidase	2.81 Å	4GSC	4GSC	4GSC	4GSC	4GSC	4GSC	Charton et al., 2014
mature peptidase	2.70 Å	4GSF	4GSF	4GSF	4GSF	4GSF	4GSF	Charton et al., 2015
mature peptidase	3.29 Å	4IFH	4IFH	4IFH	4IFH	4IFH	4IFH	Deprez-Poulain et al., 2015
mature peptidase	3.35 Å	4IOF	4IOF	4IOF	4IOF	4IOF	4IOF	McCord et al., 2013
mature peptidase	2.70 Å	4LTE	4LTE	4LTE	4LTE	4LTE	4LTE	Maianti et al., 2014
mature peptidase	3.50 Å	4M1C	4M1C	4M1C	4M1C	4M1C	4M1C
mature peptidase	2.73 Å	4NXO	4NXO	4NXO	4NXO	4NXO	4NXO	Deprez-Poulain et al., 2015
mature peptidase	2.21 Å	4PES	4PES	4PES	4PES	4PES	4PES
mature peptidase	2.33 Å	4PF7	4PF7	4PF7	4PF7	4PF7	4PF7	Durham et al., 2015
mature peptidase	2.50 Å	4PF9	4PF9	4PF9	4PF9	4PF9	4PF9	Durham et al., 2015
mature peptidase	2.21 Å	4PFC	4PFC	4PFC	4PFC	4PFC	4PFC	Durham et al., 2015
mature peptidase	3.93 Å	4Q5Z	4Q5Z	4Q5Z	4Q5Z	4Q5Z	4Q5Z
mature peptidase	3.20 Å	4QIA	4QIA	4QIA	4QIA	4QIA	4QIA	Charton et al., 2015
mature peptidase	3.15 Å	4RAL	4RAL	4RAL	4RAL	4RAL	4RAL	Liang et al., 2015
mature peptidase	2.91 Å	4RE9	4RE9	4RE9	4RE9	4RE9	4RE9	Deprez-Poulain et al., 2015
elbow-engineered-fab-bound form; complex with insulin	3.29 Å	5CJO	5CJO	5CJO	5CJO	5CJO	5CJO
crystal structure analysis of elbow-engineered-fab-bound human insulindegrading enzyme (ide)	3.80 Å	5UOE	5UOE	5UOE	5UOE	5UOE	5UOE
fab1-bound form; complex with insulin	3.95 Å	5WOB	5WOB	5WOB	5WOB	5WOB	5WOB
complex with insulin	3.70 Å	6B3Q	6B3Q	6B3Q	6B3Q	6B3Q	6B3Q
complex with fab h11-e heavy chain, fab h11-e light chain and insulin	3.70 Å	6B70	6B70	6B70	6B70	6B70	6B70
mature peptidase	6.50 Å	6B7Y	6B7Y	6B7Y	6B7Y	6B7Y	6B7Y
complex with fab h11 heavy chain and fab h11 light chain	6.50 Å	6B7Z	6B7Z	6B7Z	6B7Z	6B7Z	6B7Z
mature peptidase	6.50 Å	6BF6	6BF6	6BF6	6BF6	6BF6	6BF6
complex with fab h11-e heavy chain, fab h11-e light chain	6.50 Å	6BF7	6BF7	6BF7	6BF7	6BF7	6BF7
complex with insulin	4.20 Å	6BF8	6BF8	6BF8	6BF8	6BF8	6BF8
complex with fab h11-e heavy chain, fab h11-e light chain	7.20 Å	6BF9	6BF9	6BF9	6BF9	6BF9	6BF9
complex with insulin	3.70 Å	6BFC	6BFC	6BFC	6BFC	6BFC	6BFC
cysteine-free form; complex with substrate-selective macrocyclic inhibitor 37	2.96 Å	6BYZ	6BYZ	6BYZ	6BYZ	6BYZ	6BYZ
cysteine-free form; complex with glucagon and substrate-selective macrocyclic inhibitor 63	3.18 Å	6EDS	6EDS	6EDS	6EDS	6EDS	6EDS
cysteine-free form; complex with substrate-selective macrocycle inhibitor 63	3.57 Å	6MQ3	6MQ3	6MQ3	6MQ3	6MQ3	6MQ3
Rattus norvegicus
mature peptidase	2.08 Å	3P7L	3P7L	3P7L	3P7L	3P7L	3P7L	Noinaj et al., 2011
mature peptidase	2.14 Å	3P7O	3P7O	3P7O	3P7O	3P7O	3P7O	Noinaj et al., 2011
mature peptidase	2.27 Å	3TUV	3TUV	3TUV	3TUV	3TUV	3TUV	Noinaj et al., 2012