Fourier Shell Correlation (FSC) server
Welcome to the PDBe Fourier Shell Correlation (FSC) server!
This web service will calculate and plot the FSC curve from two 3D reconstructions that you upload.
You will be able to download the FSC curve as an XML file that can then be uploaded
as a part of a deposition to EMDB. This server uses the EMAN2
package
for the FSC calculation. The implementation of the 3 sigma and half bit threshold
curves is based on Imagic code kindly provided by Michael Schatz (ImageScience
Software GmbH).
Supported input file formats
include CCP4/MRC, Imagic, Spider,
EMAN2 HDF5 and others. We have one test data sets that you can use to try out the service
here. We appreciate your continuing
support and
feedback!
The uploaded maps will be deleted after the calculation is completed.
Quick links
Recent Entries
(Show all)
Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1B
Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1C
Local cryo-EM refinement map (Chain C N-terminal domain) for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1D
Consensus cryo-EM refinement map for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1D
Control 50S ribosomal subunit for 50S subunit structures lacking the sarcin risin loop. Class 3.
Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1C
Control 50S ribosomal subunit for 50S subunit structures lacking the sarcin risin loop. Class 1.
Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1B
Cryo-EM structure of E227Q variant of uMtCK1 in complex with transition state analog
Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1D
Control 50S ribosomal subunit for 50S subunit structures lacking the sarcin risin loop. Class 2.
Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-2D
Cryo EM reconstruction of PI3KC3-C1 in complex with Human RAB1A(Q70L), VPS34 kinase domain in the inactive conformation
Cryo-EM structure of a SUR1/Kir6.2 ATP-sensitive potassium channel in the presence of Aekatperone in the closed conformation
local refinement of arpC5, arpC1 and arp2 for arp 2/3 complex with 1 of 2 NPFs dissociated and arpC5 tail bound
Local cryo-EM refinement map (Chain D N-terminal domain) for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1D
Structure of the five-fold capsomer of the Drosophila retrotransposon Copia capsid
PI3KC3-C1 bound to RAB1A. Consensus inactive state refinement of particle subset with strongest kinase domain density
Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-2B
Local cryo-EM refinement map (Chain D N-terminal domain) for CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1B
local refinement of arpC5, arpC1 and arp2 for arp 2/3 complex 2 NPFs bound and arpC5 tail mostly dissociated
Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-2C
PI3KC3-C1 bound to RAB1A. Local refinement of VPS34KD in the inactive state, particle subset
Inactive Mu-Opioid Receptor with Nb6M, NabFab, and isoquinuclidine compound #33
PI3KC3C1 bound to RAB1A, Inactive state, VPS34 kinase domain local refinement
Cryo-EM structure of the C1q A, B-crt, C peptide assembly narrow region
PI3KC3-C1 bound to RAB1A. VPS34 kinase domain in the active conformation, consensus reconstruction
Inactive Mu-Opioid Receptor with Nb6M, NabFab, and isoquinuclidine compound#020_E1
PI3KC3-C1 in complex with RAB1A. VPS34 kinase domain active conformation
Cryo-EM structure of human uMtCK1 in complex with ADP and covalent inhibitor CKi
Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C12 and 20 mM MgCl2, State MG20-1
Structure of apo inward-open LAT1-CD98h in nanodisc, focused on TMD
Structure of LAT1-CD98hc-Fab170 in complex with JPH203, consensus map
Structure of LAT1-CD98hc-Fab170 in complex with BCH, consensus map
Cryo-EM map of the E2 inner core component of pyruvate dehydrogenase complex
Cryo-EM structure of simian rotavirus SA11 VP4 in complex with nAb 7H13-I54G mutant (left side)
Cryo-EM map of mouse subcortical maternal complex containing MATER, TLE6, FLOPED, FILIA, and SPIN1
Cryo-EM structure of simian rotavirus SA11 VP4 in complex with nAb 7H13-I54G mutant (right side)
Cryo-EM density map of trimeric Bacillus cereus CotE at intermediate resolution
Structure of apo outward-open LAT1-CD98h in nanodisc, focused on TMD
Identification, structure and agonist design of an androgen membrane receptor.
Cryo-EM structure of simian rotavirus SA11 VP4 in complex with nAb 7H13
Cryo-EM density map of hexameric Bacillus cereus CotE at intermediate resolution
Cryo-EM structure of cellodextrin phosphorylase from Clostridium thermocellum with cellodextrin ligands
Cryo-EM structure of simian rotavirus SA11 VP4 in complex with nAb 7H13 at Low concentration
Identification, structure and agonist design of an androgen membrane receptor
Identification, structure and agonist design of an androgen membrane receptor.
Identification, structure and agonist design of an androgen membrane receptor.
Identification, structure and agonist design of an androgen membrane receptor
Structure of LAT1-CD98hc in complex with melphalan, focused on TMD
Structure of apo inward-open LAT1-CD98hc-Fab170 in nanodisc, consensus map
SARS-CoV-2 EG.5.1 Spike in complex with CYFN1006-2(S-CYFN1006-2 dimer trimer).
Structure of XBB.1.16 S trimer with 2 down-RBDs complex with antibody CYFN1006-1.
Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 (state Deg1)
Comamonas testosteroni KF-1 circularly permuted group II intron Pre-1S state
Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3
Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 (state Deg3)
Comamonas testosteroni KF-1 circularly permuted group II intron Post-2S state
Structure of EG.5.1 S trimer with 3 down-RBDs complex with antibody CYFN1006-2.
Cryo-EM structure of the human ubiquitylated pre-40S ribosome with RIOK3
Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 (state Deg4)
Locally refined region of SARS-CoV-2 spike in complex with antibodies 9G11 and 3E2.
CryoEM structure of a 2,3-hydroxycinnamic acid 1,2-dioxygenase MhpB in substrate bound form
Cryo-EM structure of the human ubiquitylated 40S ribosome dimer with RIOK3
SARS-CoV-2 XBB.1.16 Spike in complex with CYFN1006-1(S-CYFN1006-1 dimer trimer).
Cryo-EM structure of the human ubiquitylated 40S ribosome with RIOK3 (state Deg2)
Cryo-EM structure of P.nat ACE2 mutant in complex with MOW15-22 RBD
Structure of SARS-CoV-2 Spike in complex with antibodies 3E2, 9G11 and 13H7.
Comamonas testosteroni KF-1 circularly permuted group II intron 2S state
Comamonas testosteroni KF-1 circularly permuted group II intron 1S state
Cryo-EM structure of the human ubiquitylated pre-40S ribosome with RIOK3 (without NOB1)
Cryo-EM structure of the reduced cytochrome bd oxidase from M. tuberculosis
The structure from subtomogram averaging of HIV-1 immature Gag with KAKA/T8I/G225R mutations
PbuCas13b Helical-2 domain in complex with anti-CRISPR protein (AcrVIB1)
Human mitochondrial ribosome in complex with antibiotic tigecycline, Class empty mtLSU body (local-filter)
NDH-PSI-LHCI supercomplex from S. oleracea: local refined peripheral arm of NDH
Cryo-EM structure of the human BRISC dimer complex bound to compound FX-171-C
Human mitoribosome purified from elatol treated HEK cells (tRNAs/mRNA at partial occupancy)
Human mitochondrial ribosome from elatol treated HEK cells (no tRNA/mRNA bound)
Human mitoribosome Class P-tRNA, mtLSU body (local filter) with tigecycline bound to mtLSU site-1 and 2
Human mitochondrial ribosome in complex with antibiotic tigecycline, Class P-tRNA consensus.
Human mitochondrial ribosome in complex with antibiotic tigecycline, Class empty consensus (local-filter)
Transcriptional activator PafBC bound to mycobacterial RNA polymerase
Human mitochondrial ribosome in complex with antibiotic tigecycline, Class empty mtSSU head (local-filter)
Cryo-EM structure of human CD163 SRCR1-9 in complex with haptoglobin-hemoglobin
Human mitoribosome Class P-tRNA, mtSSU head (local filter) with tigecycline
Human mitochondrial ribosome in complex with antibiotic tigecycline, Class A-tRNA P-tRNA mtLSU body (local-filter).
Human mitochondrial ribosome in complex with antibiotic tigecycline, Class A-tRNA P-tRNA mtSSU head (local-filter).
The structure from subtomogram averaging of HIV-1 Gag with K227A mutation
Aerolysin heptamer in membrane inserted form reconstituted in amphipoles.
The structure from subtomogram averaging of HIV-1 immature Gag with KAKA/T8I mutations
CRYO-EM STRUCTURE OF LEISHMANIA MAJOR 80S RIBOSOME : LM32Cs1C1 snoRNA overexpression
The structure from subtomogram averaging of HIV immature Gag with G225R mutation
Aerolysin double mutant K238A/K244A in styrene-maleic acid lipid particles
Composite map of the mycobacterial PafBC-bound transcription initiation complex
Sak Single Strand Annealing Protein from Staphylococcal Bacteriophage 80a - dCTD
The structure from subtomogram averaging of HIV-1 immature Gag with KAKA/G225R mutations
The structure from subtomogram averaging of HIV-1 immature Gag with A77V/KAKA/T8I/G225R mutations
Human mitochondrial ribosome in complex with antibiotic tigecycline, Class A-tRNA P-tRNA consensus.
Local cryo-EM refinement map (Chain C N-terminal domain) for CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1B
Local Refinement of VPS15 pseudokinase domain and helical repeats
Cryo-EM structure of myosin-1c bound to F-actin in the ADP-B state
Consensus cryo-EM refinement map for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1C
Structure of full-length human cardiac sodium channel - Class-II.
Kappa opioid receptor:Galphai protein in complex with inverse agonist norBNI, Original map receptor
Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-2A
Cryo-EM map of the C1q A, B-Crt, C-M10N collagenous stem assembly
Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C12 and 20 mM MgCl2, State MG20-2
Structure of the non-symmetric capsomer of the Drosophila retrotransposon Copia capsid
Locally-Refined Inactive Kappa-Opioid Receptor with Nb6M, NabFab, and isoquinuclidine compound #020_E1
Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1A
Local cryo-EM refinement map (Chain D N-terminal domain) for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1C
Locally-refined Inactive Mu-Opioid Receptor with Nb6M, NabFab, and isoquinuclidine compound #020_E1
Structure of the three-fold capsomer of the Drosophila retrotransposon Copia capsid
Cryo-EM structure of human uMtCK1 in complex with transition state analog
Local cryo-EM refinement map (Chain C N-terminal domain) for magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-1C
Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-2B
Salmonella undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (ArnC) bound to UDP
Structure of PKA phosphorylated human RyR2-R2474S in the open state in the presence of Calmodulin - focused map on xanthine
PI3KC3-C1 bound to RAB1A. Local refinement of Bara like domains of BECN1 and ATG14, and VPS15 WD40 domain
PI3KC3-C1 bound to RAB1A in the inactive state. Consensus refinement
Inactive Kappa-Opioid Receptor with Nb6M, NabFab, and isoquinuclidine compound #020_E1
Cryo-EM structure of magnesium depleted CorA in complex with conformation-specific synthetic antibody C18, State MGD-2C
Salmonella undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (ArnC)
Cryo-EM reconstruction of PI3KC3-C1 in complex with Human RAB1A(Q70L)
Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-2A
Consensus cryo-EM refinement map for CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1B
Cryo-EM structure of CorA in complex with conformation-specific synthetic antibody C18 and 100 uM MgCl2, State MG0.1-1A
Cryo-EM structure of E227Q variant of uMtCK1 incubated with ADP and phosphocreatine at pH 8.0
PI3KC3-C1 Bound to RAB1A local refinement of RAB1A/VPS34 interface