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Structural basis of a transcription pre-initiation complex on a divergent promoter.

Gorbea Colón JJ, Palao L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K
Mol Cell (2023)
Related entries: 7ui9, 7uic, 7uif, 7uig, 7uik, 7uil

Cited: 5 times
EuropePMC logo PMID: 36731470

Abstract

Most eukaryotic promoter regions are divergently transcribed. As the RNA polymerase II pre-initiation complex (PIC) is intrinsically asymmetric and responsible for transcription in a single direction, it is unknown how divergent transcription arises. Here, the Saccharomyces cerevisiae Mediator complexed with a PIC (Med-PIC) was assembled on a divergent promoter and analyzed by cryoelectron microscopy. The structure reveals two distinct Med-PICs forming a dimer through the Mediator tail module, induced by a homodimeric activator protein localized near the dimerization interface. The tail dimer is associated with ∼80-bp upstream DNA, such that two flanking core promoter regions are positioned and oriented in a suitable form for PIC assembly in opposite directions. Also, cryoelectron tomography visualized the progress of the PIC assembly on the two core promoter regions, providing direct evidence for the role of the Med-PIC dimer in divergent transcription.

Articles - 7uio mentioned but not cited (1)

  1. Structural basis of a transcription pre-initiation complex on a divergent promoter. Gorbea Colón JJ, Palao L, Chen SF, Kim HJ, Snyder L, Chang YW, Tsai KL, Murakami K. Mol Cell 83 574-588.e11 (2023)


Reviews citing this publication (3)

  1. Regulation of the RNA polymerase II pre-initiation complex by its associated coactivators. Malik S, Roeder RG. Nat Rev Genet 24 767-782 (2023)
  2. Combining per-particle cryo-ET and cryo-EM single particle analysis to elucidate heterogeneous DNA-protein organization. Palao L, Murakami K, Chang YW. Curr Opin Struct Biol 84 102765 (2024)
  3. Recent advances in infectious disease research using cryo-electron tomography. Asarnow D, Becker VA, Bobe D, Dubbledam C, Johnston JD, Kopylov M, Lavoie NR, Li Q, Mattingly JM, Mendez JH, Paraan M, Turner J, Upadhye V, Walsh RM, Gupta M, Eng ET. Front Mol Biosci 10 1296941 (2023)

Articles citing this publication (1)

  1. Mediator and Pol II go to eleven (CTD repeats). Kaplan CD. Proc Natl Acad Sci U S A 120 e2304596120 (2023)


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